本文研究了蒲公英提取物对类风湿性关节炎模型大鼠的作用机制。通过建立类风湿性关节炎模型，对大鼠进行病理学观察，并对治疗效果的相关指标如检测白介素-17（Interleukin-17，IL-17）、白介素-23（Interleukin-23，IL-23）、白介素-1（Interleukin-1，IL-1）、hsCRP、白介素-1β（Interleukin-1β，IL-1β）IL-1β、肿瘤坏死因子-α（Tumor necrosis factor-α，TNF-α）、护骨素（Osteoprotegerin，OPG）、核因子KB配体受体激活因子（RANKL、RANK）水平进行检测。研究结果表明：高剂量蒲公英组大鼠足跖肿胀度（6.42±1.06）低于模型组大鼠足跖肿胀度（15.18±2.65）（p<0.05），扭体（9.0±2.0）、缩足次数（8.5±1.5）高于模型组扭体（2.2±0.4）、缩足次数（2.4±0.5）。高剂量蒲公英组大鼠的IL-17、IL-23、IL-1、hsCRP、IL-1β、TNF-α等水平均低于模型组大鼠（p<0.01）。高剂量蒲公英组大鼠的OPG（0.42±0.06）、RANKL（0.52±0.08）、RANK蛋白表达（0.45±0.05）显著低于模型组OPG（1.18±0.15）、RANKL（1.35±0.46）、RANK蛋白表达（1.23±0.45）（p<0.01）。研究结果说明蒲公英提取物通过调控OPG/RANKL/RANK信号通路，抑制炎症因子水平表达，能显著改善风湿性关节炎模型大鼠的治疗效果。

The mechanism for dandelion extract to improve rheumatoid arthritis in rats was studied. The model of rheumatoid arthritis was established. Relevant indexes of therapeutic effect, pathological observation, and levels of interleukin-17 (IL-17), interleukin-23 (IL-23), interleukin-1 (IL-1), hsCRP, interleukin-1β (IL-β), and Tumor necrosis factor-α (TNF-α) osteoprotegerin (OPG), nuclear factor KB ligand receptor activator (RANKL, RANK) were investigated. Results showed that the swelling degree of the plantar paw in high dose of dandelion group (6.42±1.06) was lower than that in the model group (15.18±2.65) (p<0.05), the torsion (9.0±2.0) and foot contraction (8.5±1.5) were higher than those in model group (2.2±0.4 and 2.4±0.5, respectively). The levels of IL-17, IL-23, IL-1, hsCRP, IL-1β, TNF-α in high dose of dandelion group were lower than those in model group (p<0.01). The expressions of OPG (0.42±0.06), RANKL (0.52±0.08), RANK protein (0.45±0.05) in high dose of dandelion group were lower than those in model group (1.18±0.15, 1.35±0.46, 1.23±0.45, respectively) (p<0.01). The results indicated that taraxacum extract could inhibit the expression of inflammatory cytokines by regulating OPG/RANKL/RANK signaling pathway, and has a therapeutic effect on rheumatoid arthritis in rats.

河南省医学教育研究项目（Wjlx2016042）