刺梨口服液对急性醉酒小鼠的解酒护肝作用

Antialcoholism Effects of Rosa roxburghii Tratt Oral Liquid in Acute Drunkenness Mice

摘要:

本研究通过动物实验，探讨刺梨口服液对急性醉酒小鼠的解酒护肝作用。解酒作用实验：各试验组灌胃相应受试物30 d后建立急性醉酒模型，1 h后取血、肝脏，比较各组小鼠的血液乙醇浓度，并测定肝脏乙醇脱氢酶（ADH）、乙醛脱氢酶（ALDH）活力；护肝作用实验：各组于建模后12 h取血和肝脏，计算其肝脏指数，并检测小鼠血液中谷丙转氨酶（ALT）、谷草转氨酶（AST）和肝脏超氧化物歧化酶（SOD）活力、谷胱甘肽（GSH）含量。结果发现，与模型组相比，刺梨口服液高剂量组小鼠血液中乙醇浓度降低33.54%，肝脏ADH活力由26.92 U/mL上升至41.78 U/mL、ALDH活力从118.12 U/mL升高到146.72 U/mL，血清ALT、AST活力分别降低58.40%、42.69%，肝脏指数下降9.80%，肝脏SOD活力升高72.65%，肝脏GSH含量增加25.34%（p<0.05）。表明刺梨口服液具有一定的解酒作用，且对急性醉酒引起的肝损伤有明显的保护效果。

Abstract:

The antialcoholism effects and liver protection of Rosa roxburghii Tratt oral liquid in acute drunkenness mice were investigated. Anti-alcoholic experiment: Each group was established as an acute drunkenness model after all mice were administered corresponding samples for 30 days. After 1 hour, the blood and liver tissue were taken. Compared with the serum ethanol concentration, the levels of alcohol dehydrogenase (ADH) and acetaldehyde dehydrogenase (ALDH) in liver issues in different groups of mice; Liver protection experiment: The blood and liver tissue were taken after modeling. The liver index, the contents of superoxide dismutase (SOD) and glutathione (GSH) in the liver, and serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels were determined. The results showed that compared with the model group, the high-dose group of Rosa roxburghii Tratt baverage decreased the serum ethanol concentration by 33.54%. It also increased the levels of ADH and ALDH, from 26.92 U/mL to 41.78 U/mL and 118.12 U/mL to 146.72 U/mL, respectively. The serum ALT and AST contents decreased by 58.40% and 42.69%, respectively, the liver index reduced by 9.80%, and the SOD content in liver issue increased by 72.65%, the GSH content increased by 25.34% (p<0.05). It indicates that Rosa roxburghii Tratt oral liquid has the function of relieving alcohol and has obvious protective effect on acute alcohol-induced hepatic injury.