黑木耳中不同形态的铬在in vitro系统中生物可给性分析

Bioaccessibility of Different Speciation of Chromium in Auricularia auricula during the in Vitro System

摘要:

利用In vitro系统模拟黑木耳在消化过程中不同形态的铬的生物可给性，采用HPLC-ICP-MS测定不同形态铬的含量。结果表明：29个样品经过In vitro系统模拟消化后，铬在胃中的生物可给性为15.10%~35.87%；在肠中的生物可给性为11.02%~20.36%，同一样品胃中的生物可给性铬大于肠中的，且检测出的铬均以三价形态存在。可以证明在In vitro系统中，黑木耳能有效吸附对人体有害的Cr(Ⅵ)。对0.5 μg/L的Cr(Ⅲ)和Cr(Ⅵ)混标测定7次，RSD在2.55%~4.98%之间，方法重现性较好；对同一样品设置6平行实验，测量结果相对偏差均小于9.5%，说明该方法的精密度能够满足实验要求。可见，黑木耳的生物可给性铬是对人体有益的Cr(Ⅲ)，且证明了在模拟消化过程中黑木耳能够完全吸附对人体有害的Cr(Ⅵ)，为黑木耳中铬在消化过程中可能发生的形态转变及生物有效性提供了数据参考，也为研究制定菌类中铬的限量标准提供参考。

Abstract:

The in vitro system was used to simulate the digestion of Auricularia auricula in stomach and intestine to analyze the bioaccessibility of chromium. The contents of chromium with different speciations were determined by HPLC-ICP-MS. The 29 samples were simulated the digestion by in vitro system. The bioaccessibilities of chromium in the stomach were ranged from 15.10% to 35.87%, and from 11.02% to 20.36% in the intestine. The contents of chromium in stomach were more than that in the intestine. The speciation of chromium mainly was trivalent, which proved that Auricularia auricula could absorb Cr(Ⅵ). The standard solutions of 0.5 μg/L Cr(Ⅲ) and Cr(Ⅵ) were mixed to determined for seven times, which RSD was 2.55%~4.98%. Six parallel experiments were repeated to verify the precision of the method, which relative deviation of the results was less than 9.5%. It was put forward that the bioaccessibility of chromium in Auricularia auricula was Cr (Ⅲ), which was benefit for the body. The Auricularia auricula could absorb Cr(Ⅵ) which do harm to the health. This experiment explored the change of the chromium speciation in Auricularia auricula during the process of digestion in stomach and intestine, which provided a reference for the study on the limit standard of the chromium in Auricularia auricula.