Abstract:This study developed a resveratrol-grape polyphenol composite whitening oral solution, evaluated its antioxidant activity and whitening effect, and discussed preliminarily the underlying mechanism. The results of the antioxidant activity experiments showed that the whitening oral solution exhibited strong antioxidant activity: not only reduced iron ions, but also exerted significant scavenging effects on the DPPH+ and ABTS+ free radicals and peroxides. The IC50 values for DPPH+ and ABTS+ radical scavenging activities were 10.32±1.20 μg/mL and 87.06±9.52 μg/mL, respectively. The peroxyl radical scavenging and absorption capacities were 1649.00±2.78 μmol Vit. C equivalents/g and 4251.30±566.71 μmol Trolox equivalents (TE)/g, respectively. In human hepatoma cell line HepG2 model, the intracellular antioxidant activity of the whitening oral solution reached 5570.10±312.09 μmol quercetin equivalents (QE)/100 g. The mouse melanoma B16 cells, which are physiologically relevant to the human melanocytes, were selected as the research models to evaluate in vitro the whitening effect. The results showed that at a certain dose, the whitening oral solution could inhibit the proliferation of B16 cells, cellular tyrosinase activity and protein expression of B16 cells, thereby reducing the production of melanin. This composite whitening oral solution exhibited good antioxidant activity and whitening effect, thus, will have great prospects for the functional foods and healthcare products industry.
