黄连与人参协同抗氧化活性的研究

Synergistic Antioxidant Activity of Coptidis with Ginseng

摘要:

本研究比较了不同质量比的黄连提取物（CE）和人参提取物（GE）联合使用对ABTS和DPPH自由基的清除作用及其协同效果。使用药物联合作用量-效分析软件"CompuSyn"分析数据，获取不同质量比联合药物的相关参数。在ABTS体系，黄连提取物和人参提取物的IC50值分别是170.18±5.20 μg/mL和1615.73±40.17 μg/mL，联合使用黄连提取物和人参提取物（质量比4:1、2:1、1:1、1:2、1:4），IC50值分别为88.86±9.31、209.12±9.56、329.42±10.29、188.39±6.33和31.42±7.52 μg/mL。联合指数（CI）数值表明，黄连和人参提取物（4:1、2:1和1:4）都具有协同效果。在DPPH体系，黄连提取物和人参提取物的IC50值分别是67.57±9.68 μg/mL和1590.86±21.35 μg/mL，联合使用黄连提取物和人参提取物（质量比4:1、2:1、1:1、1:2、1:4），IC50值分别为49.48±12.56、195.04±7.58、129.27±15.14、404.07±10.55和153.11±6.32 μg/mL。联合指数（CI）数值表明，黄连和人参提取物（4:1和1:4）都具有协同效果。黄连和人参提取物存在协同清除ABTS和DPPH自由基效应。

Abstract:

The study compared the scavenging effects and synergies of the combinations of Coptis extract (CE) and Ginseng extract (GE) with different mass ratios on the ABTS and DPPH free radicals. The obtained data for the combinations were analyzed using the drug combined analysis software "CompuSyn" to achieve the related parameters. In the ABTS reaction system, the IC50 values of CE and GE were 170.18±5.20 and 1615.73±40.17 μg/mL, respectively, while the IC50 values of the CE-GE combinations with the mass ratios of 4:1, 2:1, 1:1, 1:2 and 1:4 were 88.86±9.31, 209.12±9.56, 329.42±10.29, 188.39±6.33 and 31.42±7.52 μg/mL, respectively. According to the combined index (CI) values, CE and GE at a ratio of 4:1, 2:1 or 1:4 exhibited synergistic effects. In the DPPH reaction system, the IC50 values of CE and GE were 67.57±9.68 and 1590.86±21.35 μg/mL, respectively, while the IC50 values of the CE-GE combinations with the mass ratios of 4:1, 2:1, 1:1, 1:2 and 1:4 were 49.48±12.56, 195.04±7.58, 129.27±15.14, 404.07±10.55 and 153.11±6.32 μg/mL, respectively. According to the combined index (CI) values, CE and GE at a ratio of 4:1 and 1:4 exhibited synergistic effects. CE and GE worked synergistically to scavenge the ABTS and DPPH free radicals.