三种蓝莓抗氧化及抗Hep G2细胞增殖活性的比较分析

Comparative Analysis of Antioxidant Activity and Anti-proliferative Activity against Hep G2 Cells of Three Blueberry Varieties

摘要:

以三种常见市售蓝莓[大兴安岭野生蓝莓（Vaccinium uliginosum L.）、贵州兔眼蓝莓（Vaccinium ashei Reade）和智利蓝莓（Vaccinium corymbosum L.）]为研究对象，研究了其总多酚、总黄酮和总花色苷含量及抗氧化活性和抗Hep G2细胞增殖活性，并分析了活性成分含量与活性功能的相关性。结果表明：大兴安岭野生蓝莓的总多酚、总黄酮和总花色苷的含量及DPPH和ABTS自由基的清除能力显著高于贵州蓝莓和智利蓝莓（p<0.05）；细胞抗氧化实验结果表明大兴安岭野生蓝莓的CAA（Cellular Antioxidant Activity）值显著高于贵州蓝莓和智利蓝莓，为202.40±6.33 μmol QE/100 g鲜果（非PBS清洗处理时）和65.40±3.04 μmol QE/100 g鲜果（PBS清洗处理时）（p<0.05）；三种蓝莓均对Hep G2细胞增殖有较强的抑制作用，其中大兴安岭野生蓝莓的抗Hep G2细胞增殖的半数有效浓度EC50显著低于贵州蓝莓和智利蓝莓，分别为150.41±7.82、212.87±13.10和221.53±12.53 mg/mL（p<0.05）；蓝莓的CAA 值与总多酚和总黄酮含量之间显著相关，抗Hep G2细胞增殖的EC50值与总多酚、总黄酮和总花色苷含量之间显著相关（p<0.05）。以上结果表明大兴安岭野生蓝莓具有极大的开发利用优势。

Abstract:

Three common commercially available blueberries (Vaccinium uliginosum L., Vaccinium ashei Reade and Vaccinium corymbosum L.) were studied for their total contents of polyphenols, flavonoids and anthocyanins, as well as the antioxidant activity and anti-proliferative activity against Hep G2 cells. The correlation between the content of active components and the activities was also examined. The results showed that the contents of total polyphenols, total flavonoids and total anthocyanins and abilities to scavenge the DPPH and ABTS free radicals of the Vaccinium uliginosum L. (a wild blueberry from the Northeast China) were significantly (p<0.05) higher than those in Vaccinium ashei Reade (a blueberry from Guizhou China) and Vaccinium corymbosum L. (a blueberry from Chile). The results of cellular antioxidant experiments indicated that the wild blueberry from the Northeast China exhibited significantly (p<0.05) higher Cellular Antioxidant Activity (CAA) values [65.40±3.04 μmol QE/100 g fresh fruit for Phosphate Buffered Saline (PBS) cleaning; 202.40±6.33 μmol QE/100 g fresh fruit for non-PBS cleaning] than the blueberries from Guizhou and the Chilean blueberry. The three types of blueberries all exhibited strong inhibitory effects on Hep G2 cell proliferation, with the half maximal effective concentration (EC50 value) of the wild blueberry from the Northeast China (150.41±7.82 mg/mL) was significantly (p<0.05) lower than those of the Guizhou blueberry (212.87±13.10 mg/mL) and Chilean blueberry (223.53±12.53 mg/mL). The CAA values of the blueberries were significantly correlated with the total contents of polyphenols and flavonoids, while there was also a significant (p<0.05) correlation between the EC50 values of anti-Hep G2 cell proliferation and the total polyphenols, and between the total flavonoid content and the total anthocyanin content. The above results indicate that the wild blueberry from the Northeast China has great advantages in development and utilization.