裂叶荨麻对Ⅱ型糖尿病小鼠肾脏损伤的保护作用

Protective Effect of Urtica fissa on Kidney Injury in Type 2 Diabetic Mice

摘要:

本文研究了裂叶荨麻对Ⅱ型糖尿病小鼠肾脏损伤的保护作用，探讨了其与抗氧化作用的关系。采用腹腔注射链脲佐菌素（STZ）构建Ⅱ型糖尿病小鼠模型。将糖尿病小鼠随机分为模型组、二甲双胍组（0.14 g/kg）、裂叶荨麻低、中、高剂量组（0.25 g/kg、0.50 g/kg、1.00 g/kg），连续灌胃4 w，检测血清尿素氮（BUN）、肌酐（SCr）水平，测定肾脏组织丙二醛（MDA）及蛋白质羰基（PCO）含量，测定超氧化物歧化酶（SOD）及过氧化氢酶（CAT）活力，观察肾脏组织病理变化。结果表明，与模型组相比，裂叶荨麻高剂量组血清BUN降低了6.15 mmol/L、SCr降低了30.87 μmol/L（p<0.05），肾脏组织MDA减少了2.97 nmol/mg、PCO含量减少了23.43 nmol/mg（p<0.05，p<0.01），SOD活性增加了55.85 U/mg、CAT活性增加了33.84 U/mg（p<0.01），缓解肾脏组织病理状态，且作用具有剂量依赖性。由此可知，裂叶荨麻对STZ诱导的Ⅱ型糖尿病小鼠肾脏损伤具有一定保护作用，其保护机制与抗氧化作用相关。

Abstract:

The study is about the protective effect of Urtica fissa on kidney injury in type 2 diabetic mice and its relationship with antioxidant activity. The model of type 2 diabetes mellitus in mice was established by intraperitoneal injection of streptozotocin (STZ). Diabetic mice were randomly divided into model group, metformin group (0.14 g/kg), low, medium and high dose group of Urtica fissa (0.25 g/kg, 0.50 g/kg, 1.00 g/kg). Mice were given saline for 4 weeks. The levels of serum urea nitrogen (BUN), creatinine (SCr) and malondialdehyde (MDA) and protein carbonyl (PCO) in kidney tissue were measured. The activity of superoxide dismutase (SOD) and catalase (CAT) were measured and the pathological changes of kidney were observed. The results showed that compared with the model group, serum BUN and SCr decreased by 6.15 mmol/L and 30.87 μmol/L (p<0.05), renal MDA decreased by 2.97 nmol/mg, PCO decreased by 23.43 nmoL/mg (p<0.05, p<0.01), SOD activity increased by 55.85 U/mg and CAT activity increased by 33.84 U/mg (p<0.01), and alleviate the pathological state of kidney tissue in a dose-dependent manner. It can be concluded that Urtica fissa has protective effect on STZ-induced kidney injury in type 2 diabetic mice, and the protective mechanism is related to antioxidant effect.