本文研究了桑叶水提物对高脂饮食小鼠粪便中胆固醇代谢产物的影响，为桑叶饮料降血脂功能确定提供数据支持。将桑叶水提物按其DNJ含量设置成高（8.0 mg/kg·bw）、中（4.0 mg/kg·bw）、低（2.0 mg/kg·bw）3个剂量组，分别灌胃高脂饮食小鼠4、8、12、16 周，测定各组小鼠粪便pH、粪固醇、胆汁酸、短链脂肪酸（Short Chain Fatty Acids，SCFAs）含量，并对SCFAs和血清胆固醇(Total Cholesterol，TC)水平、动脉粥样硬化指数（Atherosclerosis Index，AI）进行相关性分析。与高脂对照组相比，每天灌胃8 mg/kg·bw DNJ的桑叶水提物16周，小鼠粪固醇升高22.31%，胆汁酸升高22.31%，SCFAs升高36.76%，乙酸升高39.25%，丙酸升高96.52%，丁酸升高60.43%，异丁酸升高68.05%，戊酸升高74.21%，异戊酸升高23.78%，粪便pH降低2.05%，上述指标的变化均具有显著性（p<0.05）。血清TC、AI分别与乙酸、丙酸、丁酸、异丁酸、戊酸和异戊酸呈极显著负相关（p<0.01）。桑叶水提物可改善高脂饮食小鼠肠道环境，促进肠道微生物发酵产生SCFAs，促进粪固醇、胆汁酸排出，从而有助于降血脂作用。

In order to provide data support for hypolipidemic function of mulberry leaves, the effect of water extract of mulberry leaves on cholesterol metabolites in feces of high-fat diet mice was studied. The water extract of mulberry leaves was set to three dose groups of high (8.0 mg/kg·bw), medium (4.0 mg/kg·bw) and low (2.0 mg/kg·bw) according to their DNJ content. The mice were fed with high-fat diet for 4, 8, 12, and 16 weeks. The serum pH, fecal sterol, bile acid, and short-chain fatty acids (SCFAs) were measured in each group, and correlation analysis between SCFAs and serum cholesterol (Total Cholesterol, TC) levels and atherosclerosis index (AI) were analyzed. Compared with the high-fat control group, the water extract of mulberry leaves (8 mg/kg·bw DNJ) was intragastrically administered for 16 weeks, the fecal sterol increased by 22.31%, the bile acid increased by 22.31%, and the SCFAs increased by 36.76%, acetic acid increased by 39.25%, propionic acid increased by 96.52%, butyric acid increased by 60.43%, isobutyric acid increased by 68.05%, valeric acid increased by 74.21%, isovaleric acid increased by 23.78%, and fecal pH decreased by 2.05%. The changes in the indicators were all significant (p<0.05). Serum TC and AI were significantly negatively correlated with acetic acid, propionic acid, butyric acid, isobutyric acid, valeric acid and isovaleric acid (p<0.01). Mulberry leaf water extract can improve the intestinal environment of mice with high-fat diet, promote intestinal microbial fermentation to produce SCFAs, promote the excretion of fecal sterol and bile acid, and thus help reduce blood fat.

现代农业产业技术体系建立专项（CARS-18）；公益性行业（农业）科研专项（201403049）；重庆市科委社会民生重点项目（CSTS2017shms-zdyfx0054）