辣木多肽对D-半乳糖致衰老小鼠抗氧化功能的影响

Effect of Polypeptide from Moringa oleifera on Antioxidant Capability in D-galactose Induced Aging Mice

摘要:

探讨辣木多肽(MOPP)对D-半乳糖致衰老小鼠抗氧化能力的影响。小鼠腹腔注射D-半乳糖(120 mg/kg)建立衰老模型。建模成功后，分设正常组，D-半乳糖衰老组，维生素C组，MOPP低剂量(10 mg/kg)和高剂量(100 mg/kg)组。连续给药6周后，依试剂盒说明分别测定血清总抗氧化力(T-AOC)，脑、心脏和肝脏及血清中超氧化物歧化酶(superoxide dismutase，SOD)，过氧化氢酶(catalase，CAT)，谷胱甘肽过氧化酶(glutathione peroxidase，GSH-Px)和丙二醛(malondiadehycle，MDA)水平。qRT-PCR法测定肝内Mn-SOD、Gu/Zn-SOD和CAT的mRNA表达。与衰老对照组相比，MOPP能分别有效提高衰老小鼠血清T-AOC能力(至13.73~17.98 U/mL)，及血清SOD(8.04~11.65 U/mg protein)、CAT(4.56~6.45 U/mg protein)和GSH-Px(7.28~10.65 U/mg protein)活性，并显著提升脑、心脏及肝组织中抗氧化物酶(SOD、CAT、GSH-Px)活力(p<0.05)。同时，高浓度MOPP还能使血清、脑、心脏和肝组织中MDA水平显著降低43.6%，44.7%，31.4%和56.0%(p<0.05)。此外，MOPP干预还能增强肝脏中SOD、CAT和GSH-Px的mRNA转录。本研究结果提示MOPP能明显提高D-半乳糖致老龄小鼠体内的抗氧化状态。

Abstract:

To investigate the effect of polypeptide from Moringaoleracea (MOPP) on the antioxidant capability of D-galactoseinduced aging mice, the aging model was established by intraperitoneal injection of D-galactose (120 mg/kg) in mice. The model mice were divided into normal group, D-galactose aging group, vitamin C group, MOPP low dose (10 mg/kg) and high dose (100mg/kg) group. After 6 weeks of continuous administration, the serum level of total antioxidant capacity (T-AOC), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), as well as malondiadehycle (MDA) in serum and main organs including brain, heart and liver were determined by commercial assay kits. The mRNA expressions of Mn-SOD, Gu/Zn-SOD and CAT in liver were determined by qRT-PCR assay. Compared with the aging group, MOPP treatment could significantly improve the serum levels of T-AOC (to 13.73~17.98 U/mL) and activity of SOD(8.04~11.65 U/mg protein), CAT (4.56~6.45 U/mg protein) and GSH-Px (7.28~10.65 U/mg protein) in aging mice. MOPP treatment also increase the activities of SOD, CAT and GSH-Px in main organs (brain, heart and liver) of aging mice (p<0.05). High dose of MOPP also reduced the contents of MDA in the serum, brain, heart and liver revealed a reducing trend by 43.6%, 44.7%, 31.4% and 56.0% (p<0.05), respectively. In addition, MOPP treatment enhanced mRNA transcription of antioxidant factors (SOD, CAT, GSH-Px) in liver of aging mice. The results of this work suggested that MOPP could significantly improve the antioxidant status of D-galactose-induced mice.