本文研究红豆越橘（Vaccinium vitis-idaea L.）不同溶剂萃取物对炎症小鼠免疫功能及抗炎的影响。昆明小鼠按体重10 mg/kg剂量灌胃，连续灌胃15 d，测定小鼠血清、肝脏中谷胱甘肽（glutathione，GSH）、丙二醛（malondialdehyde，MDA）的含量和总超氧化物歧化酶（total superoxide dismutase，T-SOD）、谷胱甘肽还原酶（glutathione reductase，GR）活性。建立耳肿胀炎症模型，分析不同溶剂萃取物对耳肿胀抑制情况。建立LPS脓毒症模型，采用酶联免疫ELISA试剂盒法测量PGE2及NO含量变化。结果显示，四组给药组均能不同程度地提高抗氧化酶活力、增加GSH的含量、降低MDA含量。其中血液中正丁醇组GSH含量最高，提高了33.47%，肝脏中石油醚组GSH含量最高，提高了38.61%；乙酸乙酯组能够显著提高血液中35.75%的T-SOD活力、降低27.97%的MDA含量；正丁醇组可以显著增加44.44%和35.68%血液和内脏中的GR活性；四组给药组能够显著抑制LPS诱导的NO和PGE2产生，乙酸乙酯组略低于阳性对照地塞米松组。本实验证实，红豆越橘萃取物不仅可以有效提高机体中抗氧化酶的水平，起到保护细胞的作用，而且可以减少由于氧化应激炎症产生的PGE2和NO。为红豆越橘的综合开发提供理论依据。

In this work, the effects of different solvent extracts of Vaccinium vitis-idaea L. on the anti-inflammatory and immune functions of inflammatory mice were studied. Continuous oral gavage for 15 days at doses of 100 mg/kg body weight was conducted. The content of glutathione (GSH), malondialdehyde (MDA) and total superoxide dismutase (T-SOD), glutathione reductase (GR) in serum and liver of mice were measured. An ear swelling inflammation model was established to evaluate the inhibition of ear swelling by different solvent extracts. The sepsis model LPS was established to investigate the change in the content of NO and PGE2 by enzyme-linked immunoassay ELISA kit method. The results showed that, for all the four groups treatment, the activity of antioxidant enzymes and the content of GSH increased, whlie the content of MDA decreased. Among them, the GSH content in the n-butanol group was the highest, which increased by 33.47%. The GSH content in the petroleum ether group was the highest in the liver, which increased by 38.61%. For the ethyl acetate group, the T-SOD activity in the blood increase significantly and the MDA decreased by 27.97%. For the content of n-butanol group, the activity of GR in blood and viscera increase significantly by 44.44% and 35.68%, respectively. For all the four groups, the production of NO and PGE2 induced by LPS coukd be inhibited significantly. The effect of inhibition of the ethyl acetate group was slightly worse than the positive control dexamethasone group. This experiment confirmed that Lingonberry extract can not only effectively increase the level of antioxidant enzymes in the body, but also protect cells. It can also reduce PGE2 and NO caused by oxidative stress inflammation. This work provided a theoretical basis for the comprehensive development of lingonberry.

黑龙江省科学自然基金项目（QC2016021）；中国博士后科学基金项目（31170510）；中央高校科学前沿与交叉学科创新基金项目（2572016CA06）；国家自然科学基金项目（31170510）