芦荟多糖联合低聚果糖缓解小鼠酒精性肝损伤

Aloe Polysaccharide Combined with Fructose-oligosaccharide in the Treatment of Alcoholic Liver Injury in Mice

摘要:

本文研究了芦荟多糖联合低聚果糖对慢性酒精所致小鼠肝损伤的保护作用。采用芦荟多糖联合低聚果糖灌胃，以保护慢性酒精性肝损伤小鼠，实验结束后测定小鼠肝脏指数；血清生化指标，如谷丙转氨酶(ALT)、谷草转氨酶(AST)、总胆固醇(TC)和甘油三酯(TG)；肝脏生化指标，如还原型谷胱甘肽(GSH)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、肿瘤坏死因子(TNF-α)和白介素-6(IL-6)，同时观察组织病理学变化，以综合评价芦荟多糖联合低聚果糖对小鼠的肝保护作用。结果表明，高剂量组芦荟多糖联合低聚果糖能显著地降低酒精诱导的ALT、AST、TC、TG的水平至(23.37±0.80) U/L、(106.50±4.58) U/L、(2.85±0.10) mmol/L、(1.38±0.12) mmol/L；降低炎症因子IL-6和TNF-α水平至(28.24±0.45)和(155.90±9.67) pg/(mg pro)和提升丙二醇MDA含量至(1.25±0.13) pmol/(mg pro) (p<0.05)，并且提高肝内抗氧化酶GSH、SOD、GSH-Px活性至(93.65±4.93)、(61.24±3.13)和(82.75±7.04) U/(mg pro) (p<0.05)，高剂量组的肝保护效果较阳性药物水飞蓟更明显，其主要保护机制为抗氧化。因此，本研究为缓解酒精性肝损伤的芦荟多糖联合低聚果糖复方产品提供了一定的理论支撑。

Abstract:

The protective effects of aloe polysaccharide combined with fructose oligosaccharide on liver injury induced by chronic alcohol in mice were investigated. Aloe polysaccharide combined with fructose oligosaccharide was used to protect mice from chronic alcoholic liver injury. The liver index of mice was measured. Serum biochemical indexes, such as alanine aminotransferase (ALT), aspartate aminotransferase (AST), total cholesterol (TC) and triglyceride (TG), and liver biochemical indexes such as reduced glutathione (GSH), superoxide dismutase(SOD), glutathione peroxidase (GSH-PX), tumor necrosis factor (TNF-α) and interleukin-6 (IL-6), were analyzed to to evaluate the protective effect of aloe polysaccharide combined with fructose oligosaccharide on liver in mice. The histopathological changes were also observed. The results showed that aloe polysaccharide combined with fructose oligosaccharide could significantly decrease ALT、AST、TCand TGto (23.37±0.80) U/L, (106.50±4.58 ) U/L (2.85±0.10) mmol/L, (1.38±0.12) mmol/L, and increase the antioxidant enzyme GSH、SOD、GSH-Px inliver to (93.65±4.93), (61.24±3.13) and (82.75±7.04) U/(mg pro) (p<0.05), respectively. However, the levels of IL-6 and TNF- α decreased to (28.24 ±0.45) and (155.90±9.67) pg/(mg pro), respectively. The protective effect of liver in high dose group was more obvious than that of silythistle. Its main protective mechanism was antioxidation. Therefore, this work provided a theoretical support for Aloe polysaccharide combined with fructose oligosaccharide compound to alleviate alcoholic liver injury.