1-MCP、乙烯调控杏果采后活性氧代谢酶活性及同工酶电泳的研究

Activities of Active Oxygen Metabolism Enzymes and Isoenzymes in Apricot Fruit Treated with 1-MCP and Ethephon

摘要:

以轮台白杏为试材，使用1 μL/L1-甲基环丙烯（1-MCP）和30 mg/kg乙烯利处理，研究25 ℃贮藏过程中，杏果果肉中超氧化物歧化酶(SOD)、多酚氧化酶(PPO)、过氧化物酶(POD)和脂氧合酶（LOX）的活性变化规律及其同工酶表达变化。研究表明：1-MCP处理组显著地延缓了杏果衰老软化，乙烯利处理加速了杏果衰老软化；经1-MCP处理后的杏果SOD、PPO、POD的酶活均高于对照组及乙烯利处理组。SOD活性比对照组高25.21%（p<0.05）、PPO活性比对照组高12.93%（p<0.05）、POD活性比对照组高9.96%（p<0.05），LOX活性显著低于对照及乙烯利处理组，比对照组低46%（p≥0.05）；1-MCP处理组、对照较乙烯利处理的SOD、PPO、POD同工酶表达增强，LOX同工酶表达减弱。随着贮藏时间的增加同工酶条带由清晰变为弥散，同工酶表达随着时间的增加而减弱。1-MCP通过提高活性氧代谢相关酶的活力，减少活性氧的产生，减缓细胞衰老进程，延缓软化，提高杏果的贮藏品质。

Abstract:

Using Luntai Baixing as test material, 1 μL/L of 1-methylcyclopropene (1-MCP) and 30 mg/kg of ethephon were used to treat apricot fruit, the aim is to study the changes in the activities of superoxide dismutase (SOD), polyphenol oxidase (PPO), peroxidase (POD) and lipoxygenase (LOX) in apricot pulp stored at 25 ℃, and their isoenzyme expression changes. The results showed that the 1-MCP treatment group significantly delayed the aging and softening of apricot fruit, and the ethephon treatment accelerated the senescence and softening of apricot fruit. The enzyme activities of SOD, PPO and POD in the apricot fruit treated by 1-MCP were higher than those in the control and ethephon treatment groups. The activity of SOD was 25.21% higher than that of the control group (p<0.05), the activity of PPO was 12.93% higher than that of the control group (p<0.05), the activity of POD was 9.96% higher than that of the control group (p<0.05), and the activity of LOX was significantly lower than that of the control group, and the ethephon treatment group was 46% lower than the control group (p≥0.05). The expression of SOD, PPO and POD isoenzymes in the 1-MCP treatment group and the control group were enhanced, and the expression of LOX isoenzyme was reduced. As the storage time increased, the isozyme bands changed from clear to diffuse band, and isozyme expression decreased with time. 1-MCP reduced the activity of active oxygen metabolism and the production of reactive oxygen species, slowed down the process of cell aging, delayed softening, and improved the storage quality of apricot fruit.