UPLC-MS-MS同时测定茶油中8种儿茶素类物质

Determination of 8 Catechins Phenolic Compounds in Camellia Oil with Ultra High Performance Liquid Chromatography Tandem Mass Spectrometry

摘要:

利用超高效液相色谱-串联质谱建立了一种可以同时检测茶油8种儿茶素类物质的分析方法。使用Waters T3 C18色谱柱(250 mm×4.6 mm，5 μm)，以0.1%（V/V）甲酸水溶液和甲醇和为流动相进行梯度洗脱，正扫描方式下多反应监测(MRM)模式测定。在优化的条件下，8种儿茶素类物质线性关系良好（r2=0.9966~0.9995），方法检出限为0.2~1.1 μg/L。在不同浓度水平的添加平均加标回收率为76.4.%~93.4%之间,相对标准偏差3.0%~8.9%。应用本方法可以检测出茶油中儿茶素(C)、表儿茶素表儿茶素(EC)、表没食子儿茶素(EGC)、表儿茶素没食子酸酯(ECG)、表没食子儿茶素没食子酸酯(EGCG)等的含量。结果表明，茶油中表没食子儿茶素没食子酸酯(EGCG)、表没食子儿茶素(EGC)、表儿茶素没食子酸酯(ECG)的含量相对较高。本方法结果准确可靠，方法快速，简单易行，可为茶油中儿茶素类物质的检测提供参考。

Abstract:

A rapid method was developed for the simultaneous determination of catechins phenolic compounds in Camellia oil by ultra high performance liquid chromatography coupled with tandem mass spectrometry (UPLC-MS/MS). The compounds were separated on a waters T3 C18 column with 0.1% (V/V) formic acid aqueous solution and methanol as mobile phase by gradient elution, then analyzed by positive electrospray ionization tandem mass spectrometry under multiple reaction monitoring (MRM) mode. Under the optimal conditions, 8 catechins phenolic compounds showed good linearity over tested concentration ranges with the correlation coefficients from 0.9966 to 0.9995 (r2). The 1imits of detection (LOD) were in the range of 0.2~1.1 μg/L. The average recoveries of different levels ranged from 76.4% to 93.4% with the relative standard deviations (RSDs) from 3.0% to 8.9%. This method was employed to detect 8 phenolic compounds including C, EC, EGC, ECG, EGCG in Camellia oil. The results indicated that the EGCG is much higher in Camellia oil. The method was accurate and reliable, quick and suitable for the analysis of phenols in edible oils.