Abstract:In this study, was substituted with, and subsequently, the effects of substituting low gluten-wheat flour (20%~80%, W/W) with whole barley flour (hereinafter referred to as barley flour) on the gelatinization properties of mixed flour, dough textural characteristics and baking quality of crackers. The results revealed that with the increase in the amount of barley flour added, the peak viscosity, trough viscosity, final viscosity, setback value and gelatinization temperature of the barley-wheat mixed flour increased significantly, while the dough’s hardness, resilience, gumminess and chewiness increasing significantly with the adhesiveness, cohesiveness and springiness decreasing significantly. The baking qualities of the mixed flour crackers changed after the addition of barley flour: The higher amount of adding barley flour, the darker the cracker and the higher the dietary fiber content; The cracker hardness increased gradually with the increase in the amount of barley flour for addition, and reached the peak value when the addition amount was 60%. The shape, mouthfeel, color and overall score all decreased continuously and the overall score dropped sharply, when the amount of barley flour added exceeded 60%. Therefore, the addition of barley flour is beneficial to increase the content of dietary fiber in crackers, but not conducive to the stability of dough, and the mouthfeel of barley bran and its dark color are still the key factors affecting the acceptance of whole barley processed foods.