采用体外细胞实验和体内动物实验研究余甘子提取物(PLE)对对乙酰氨基酚(APAP)诱发的的肝损伤的保护效果和相关作用机制。检测了小鼠血清中的谷草转氨酶(AST)和谷丙转氨酶(ALT)水平、肝匀浆谷胱甘肽(GSH)和丙二醛(MDA)水平、肝组织病理检测；Westem blot检测核转录因子E2相关因子2 (Nrf2)的表达；PCR检测mNqo1、mG6pdx、mSOD2的表达。肝细胞HepG2实验表明，PLE组与APAP组相比，经过PLE处理的肝细胞可以保持较高的细胞活力及GSH含量，PLE处理组GSH的含量也显著提高至16.91±4.34和25.19±1.33 μmol/mg。动物实验表明PLE含量越高可以将ALT、AST水平降低越明显，PLE处理组血清ALT和AST分别为263.7±87.1 IU/L，188.4±45.9 IU/L和640.9±224.6 IU/L，155.9±50.6 IU/L，不仅可以降低肝细胞MDA表达并且可以提高GHS含量，并使Nrf2转位入核，下游相关基因mNqo1、mG6pdx、mSOD2的表达也明显上升。总之高浓度的PLE可以针对APAP引起的肝损伤对肝组织进行保护，该保护作用可能与激活抗氧化应激Nrf2/ARE信号通路有关。

Hepatic cells and in vivo mice assay were used to investigate the potential protective mechanisms of PLE (Phyllanthus emblica L. extracts) against acetaminophen (APAP)-induced hepatotoxicity. The levels of serum alanine aminotransferase (ALT), aspartate aminotransferase (AST) were detected. The contents of glutathione (GSH) and malondiadehyde (MDA) in liver tissue were measured by commercial kits. HE staining was performed to observe morphological changes of the liver. The protein expression of Nrf2 was detected by Western blotting. The mRNA expressions of mNQO1, mG6pdx, mSOD2 was tested by quantitative real-time PCR. The results showed that in HepG2 cells, APAP exposure induced the decrease of the cell viability and GSH content, which were both greatly restored by PLE pretreatment. The content of GSH was increased to 16.91 ± 4.34 and 25.19 ± 1.33 μmol/mg, respectively by PLE treatment. Compared with the model group, the serum activities of ALT and AST as well as MDA content remarkably reversed by the administration of PLE (100 mg/kg ), while GSH contents were elevated in liver tissues. The content of ALT and AST decreased to 263.7±87.1 IU/L, 188.4±45.9 IU/L and 640.9±224.6 IU/L, 155.9±50.6 IU/L, respectively. Nrf2 protein expression in the liver tissue as well as mRNA expressions of mNQO1, mG6pdx, mSOD2 increased. High-dose PLE can inhibit APAP-induced hepatotoxicity, and the mechanism might be associated with the activation of Nrf2 /ARE signaling pathway.

江西中医药大学重点学科支持青年教师培养计划项目(2017JZZDXK004)；江西省卫生计生委科技计划项目(20185321)；江西省教育厅科技计划项目资助(GJJ180694)