辣木籽多酚的分离纯化研究

Separation and Purification of Polyphenols in Moringa Seeds

摘要:

辣木籽多酚已经被证明有极好的抗氧化功能，为了更好的研究辣木籽多酚的生物活性及理化性质，对提取出的粗提物进一步的分离和去除杂质非常有必要。本研究对超声辅助提取的辣木籽多酚经一步纯化，测定了五种不同类型的大孔树脂中辣木籽多酚的吸附率、解吸率，筛选出分离纯化辣木籽多酚的最佳大孔树脂，采用静态、动态吸附解吸实验研究辣木籽多酚分离纯化的适宜条件。结果表明：D-101大孔树脂效果最好并确定最优的吸附，解吸条件为：样品液pH为5，上样液浓度为3 mg/mL，洗脱液为80%的乙醇溶液，上柱流速为2 mL/min，洗脱流速为2 mL/min，在此条件下得到的辣木籽多酚的纯度由10.37%提高到32.29%。纯化效果明显，对辣木籽多酚的分离纯化提供了指导意义。

Abstract:

Moringa seed polyphenols have been proven to possess excellent antioxidant properties. In order to study the bioactivity and physicochemical properties of Moringa seed polyphenols, it is necessary to further separate and remove impurities from the crude extract of Moringa seeds. In this study, the crude extract obtained via ultrasonic-assisted extraction was subjected to further purification. The adsorption rate and desorption rate of the polyphenols from Moringa seeds on different types of macroporous resins were determined, to screen the most suitable macroporous resin for separating and purifying polyphenols. The optimal conditions for the separation and purification of Moringa seed polyphenols were studied by static and dynamic adsorption and desorption experiments. The results showed that D-101 macroporous resin performed the best (which was used to determine the optimal adsorption and desorption conditions). The optimal conditions were: pH and concentration of the sample solution, 5 and 3 mg/mL; Eluent, 80% ethanol solution; olumn flow rate, 2 mL/min; Elution flow rate, 2 mL/min. The purity of the Moringa seed polyphenol extract increased from 10.37% to 32.29%, after the extract was treated under such conditions. The purification effect was significant, thus the findings of this study provided guidance for the separation and purification of Moringa seed polyphenols.