探讨葛枳口腔崩解片对醉酒小鼠的解酒作用及对酒精性肝损伤小鼠的保肝作用的影响。抗醉酒研究：造模后采用葛枳口腔崩解片高、中、低剂量组进行灌胃干预，以纯净水作为对照，记录翻正反射消失和恢复时间。保肝作用研究：除正常组灌胃纯净水外、其余各组每天灌胃56度白酒，30 min后除空白组和模型组灌胃纯净水外，阳性药物及高、中、低剂量组灌胃各药物，连续10 d。测量小鼠的体重变化并计算其肝脏系数，检测血清中谷丙转氨酶（ALT）、谷草转氨酶（AST）及肝脏组织中超氧化物歧化酶（SOD）、丙二醛（MDA）、谷胱甘肽（GSH）含量。与模型组相比，药物高剂量组将小鼠醉酒时间延长了6 min，醒酒时间缩短了122.94 min (p<0.01)，药物组可不同程度的增加小鼠体重并降低肝脏质量指数。与正常组比，模型组血清ALT含量增加了99.53 IU/L，AST含量增加了48.03 IU/L，肝组织中MDA升高了9.45 nmol/mg，肝组织中GSH含量降低了55.87 nmol/g，SOD含量降低了51.13 U/mg (p<0.05)。与模型组比，药物各剂量组可降低血清ALT、AST及肝组织中的MDA含量，升高肝脏GSH、SOD含量(p<0.05),结果呈剂量依赖关系。由此可知，葛枳口腔崩解片具有解酒保肝作用。

Zhige oral disintegration tablets’ relieving effect on the hangover of drunken mice and hepatoprotective effect on mice with alcoholic liver injury were investigated. In anti-drunk study, after the model was established, 3 groups of mice were given intragastric intervention with high, medium and low dose of Zhige oral disintegration tablets, pure water was used as a control, and the time for the disappearance of righting reflex and recovery was recorded. In liver protection study, except the normal group was given pure water, the other groups were given 56-degree white spirits, and after 30 minutes, except thenormal group and the model group, the positive drug group and the high, medium and low dose groups were administered bifendate and Zhige oral disintegration tablets respectively for 10 consecutive days. The body weights of the mice were measured and their liver coefficients were calculated. The serum alanine aminotransferase (ALT), aspartate aminotransferase (AST) and superoxide dismutase (SOD), malondialdehyde (MDA) and glutathione (GSH content) in the liver tissues were measured. Compared with the model group, the drunken time and the hangover time of the mice in the high-dose group were prolonged by 6 min and 122.94 min (p<0.01), respectively. In the drug groups, the body weight of the mice increased and the liver mass indexes decreased to various degrees. Compared with the normal group, the model group’s serum ALT level increased by 99.8 IU/L, AST content increased by 48.03 IU/L, MDA of the liver tissue increased by 9.45 nmoL/L, and GSH content of the liver tissue decreased by 9.45 nmoL/mg, and SOD content was reduced by 51.13 U/mg (p<0.05). Compared with the model group, the high, medium and low dose groups’ serum MALT, AST and MDA content in liver tissue were reduced and liver GSH and SOD content (p<0.05) were increased, and the results were dose-dependent. It could be seen that Zhige oral disintegrating tablets were alcohol relieving and hepatoprotective.

国家自然科学基金项目（81803698）；陕西省自然科学基础研究计划项目（2017JM8079）；陕西省教育厅专项科研计划项目（16JK1089）