L-阿拉伯糖促进秀丽隐杆线虫的生长发育及降低脂肪合成

L-arabinose Promotes the Growth and Development of Caenorhabditis elegans and Reduces Fat Synthesis

摘要:

本文以秀丽隐杆线虫作为模式生物，研究L-阿拉伯糖对其生长发育及脂肪合成能力的影响。试验采用葡萄糖作为对照组，分别添加浓度为2.5、5、7.5、10、20 mmol/L的L-阿拉伯糖作为试验组，将线虫进行同步化处理后，分析线虫头部摆动频率、身体弯曲频率、身体长度、子代数目及其甘油三酯含量和油红O染色结果。结果显示，在现有添加浓度下，L-阿拉伯糖对秀丽隐杆线虫的身体弯曲频率、子代数目无显著影响，但对线虫的头部摆动频率、身体长度影响显著：当浓度为20 mmol/L时，线虫头部摆动频率相对于对照组增加了约20%；当浓度为7.5 mmol/L时，线虫身体长度与对照组相比增加了约11%；甘油三酯分析结果显示，L-阿拉伯糖试验组中线虫体内的甘油三脂含量均低于对照组，并随添加浓度的增加显著降低（p<0.01），当L-阿拉伯糖浓度为20 mmol/L时，线虫体内甘油三酯含量与对照组相比降低了约67%；油红O染色结果与甘油三酯分析结果一致。本试验研究表明，L-阿拉伯糖能够促进秀丽隐杆线虫的头部摆动频率和身体生长，并对于降低其脂肪合成作用具有较为明显的效果，具有一定的降脂功效。

Abstract:

In this work, the effect of L-arabinose on the growth development and fat synthesis capacity of Caenorhabditis elegans was investigated. Glucose was used as control sample while 2.5, 5.0, 10.0 and 20.0 mmol/L of L-arabinose were added respectively as treatment groups. After pretreatment of synchronized, the head swing frequency, body bending frequency, body length, number of offspring, triglyceride content and oil red O staining of elegans were analyzed. The results showed that L-arabinose had no significant effect on the body bending frequency and number of offspring of Caenorhabdits elegans. However, it had a significant efficiency on the head swinging frequency and body growth of elegans. When the concentration was 20 mmol/L, the head swining frequency increased by 20%. When the concentration of L-arabinose was 7.5 mmol/L, the body length of elegans increased by 11%. The triglyceride analysis revealed that the contents of triglyceride in treatment groups were lower than that of control group and decreased gradually with increasing L-arabinose concentrations. When the concentration was 20 mmol/L, the triglyceride content in elegans reduced by nearly 67% compared with the control group. The results of oil red O staining were consistent with the analysis of triglyceride. Results indicated that L-arabinose can promote the head swining frequency and body growth of Caenorhabdits elegans and has obvious effect on reducing fat synthesis of them, thus has a certain lipid-lowering function.