响应面法优化香水莲花多酚的提取工艺及其抗氧化活性

Extraction Optimization through Response Surface Methodology and Antioxidant Activity of Polyphenols of Nymphaea Hybrid

摘要:

为了研究香水莲花多酚提取工艺的最佳条件及其抗氧化能力，本文采用响应面分析法优化香水莲花多酚的提取工艺，以香水莲花多酚提取率为指标，对浸提温度、液料比、浸提时间以及乙醇浓度进行了单因素试验，并根据Box-Behnken试验原理，进行四因素三水平的响应面法优化；同时，以香水莲花多酚清除DPPH自由基、ABTS自由基，及FRAP还原力为指标，来评价其抗氧化性。研究结果表明：香水莲花多酚的最优提取条件是：浸提温度为58 ℃，液料比为48:1（mL/g），浸提时间为1.9 h，乙醇浓度为57%，在该条件下香水莲花多酚提取率可达8.28%。香水莲花多酚对DPPH自由基的清除率可达90.22%，对ABTS自由基的清除率达到88.01%，FRAP还原力则随多酚浓度的升高而增大，且呈一定的量效关系，表明香水莲花多酚的抗氧化作用较强。

Abstract:

In order to explore the best extraction technology and antioxidant activity of polyphenols from Nymphaea hybrid, the optimization of polyphenols extraction conditions was researched by the method of response surface methodology. The influences of extraction temperature, liquid/solid ratio, extraction time, and alcohol concentration on polyphenol extraction yield were researched by single factor experiments. Based on Box-Behnken, four-factor and three-level response surface methodology was carried out to optimize the extraction technology. The antioxidant activity of polyphenols was evaluated using DPPH and ABTS radical scavenging activities and FRAP reducing power. The results found that the optimum conditions for polyphenol extraction were obtained as follows: extraction temperature of 58 ℃, liquid/solid ratio of 48:1 (mL/g), extraction time of 1.9 h, and alcohol concentration of 57%. Under the optimal conditions, extraction rate of N. hybrid polyphenols was 8.28%. The DPPH and ABTS scavenging abilities of N. hybrid polyphenols were 90.22% and 88.01%, respectively. The FRAP value was increased with the polyphenol concentration, exhibiting a dose-dependent effect. These results suggested that N. hybrid polyphenols has an obvious antioxidant activity.