玛咖生物碱对人肝癌细胞Bel-7402和H22荷瘤小鼠的抑制作用

Inhibition of Bel-7402 Human Hepatoma Cells and H22 Tumor-bearing Mice by Maca Alkaloids

摘要:

探究玛咖生物碱对Bel-7402细胞体外抗肝癌活性及体内抗H22肝癌活性的影响。通过0.5%的盐酸95%乙醇溶液提取获得玛咖生物碱粗提物，并通过进一步的酸溶碱沉方法纯化获得玛咖生物碱，通过MTT法测定不同剂量玛咖生物碱对Bel-7402细胞抑制率的影响，并进一步通过体内抗肝癌H22荷瘤小鼠试验，考察不同剂量玛咖生物碱体内抗肝癌活性影响。细胞实验表明，不同剂量组玛咖生物碱对Bel-7402细胞有一定的抗肝癌活性，其抗癌活性高低顺序为：玛咖生物碱高剂量组>玛咖生物碱中剂量组>玛咖生物碱低剂量组。体内抗肝癌试验表明，玛咖生物碱高、中剂量组具有显著的抗肝癌活性。可见，玛咖生物碱具有一定的抗肝癌活性，这为玛咖生物碱活性的研究提供新的方向。

Abstract:

The effects of maca alkaloid on the anti-hepatocarcinoma activity of Bel-7402 cells in vitro and the anti-H22 liver cancer activity in vivo were investigated. Maca alkaloid crude extract was obtained by 0.5% hydrochloric acid and 95% ethanol solvents, and maca alkaloid was further purified by acid dissolution and alkali precipitation method. The anti-hepatocarcinoma activity of maca alkaloids was determined by MTT method and the anti-H22 liver cancer activity in vivo. The cell experiments showed that maca alkaloids of different doses exhibited an anti-liver cancer activity towards Bel-7402 cell, and the decreasing order of the activity was maca alkaloids high-dose group > maca alkaloids middle-dose group > maca alkaloids low-dose group. The in vivo anti-liver cancer results showed that maca alkaloids at high or middle doses had a significant anti-liver cancer activity. The results demonstrated that maca alkaloids exhibit an anti-liver cancer activity, which would provide a new direction for the study of maca alkaloid activity.