Abstract:Microorganisms from the production process of bottled drinking water plants distributed in A, B, C, D four cities were investigated, to obtain basic microbial contamination data, and evaluate the effectiveness of the microbial contamination control system for the production process of the plants. Microbial analyses of raw water, process water and finished water from the production process of four factories were carried out, and the dominant strains were identified by traditional and molecular identification techniques. The obtained results revealed that the number of molds and yeasts or coliforms of 33 samples was less than 1 CFU/mL. The number of total bacterial colonies in sand-filtering water and carbon-filtering water was detected and the detection values were high. Fecal streptococcus was detected in the process water through a carbon filter, and Pseudomonas aeruginosa was detected in the Reverses Osmosis (RO) water, although Clostridium perfringens was not detected in either water. A total of 34 strains of bacteria were isolated, and dominant bacteria included Acidovorax sp., Pseudomonas sp., Mycobacterium sp., and Bacillus sp.. Microbial monitoring in the production process of bottled drinking water is reasonably effective and the microbial risk is low. It is suggested that the bottled drinking water plants should establish the in-process microbial identification system in the production, strengthen microbial supervision, monitor the microbial risk in the production process, and provide scientific basis for the rational and effective control of microbial risk in the production process.
