[关键词]
[摘要]
以阿克苏富士苹果为试材,通过测定在贮藏过程中(0~150 d),果实蔗糖、葡萄糖、果糖和山梨醇含量,以及蔗糖磷酸合成酶(SPS)、蔗糖合成酶(SS-S、SS-C)、酸性转化酶(AI)、中性转化酶(NI)和山梨醇脱氢酶(SDH)的活性,研究采后苹果果实糖代谢及相关酶活性的变化规律并分析了1-MCP处理对它们的影响。结果表明:在采后贮藏期间1-MCP处理在一定程度上抑制了贮藏过程中富士苹果果实蔗糖、葡萄糖、果糖和山梨醇含量的增加,且对不同采收期果实酶活性的影响效果不同,果实中蔗糖、山梨醇含量以及SDH活性呈下降趋势,果糖含量、葡萄糖含量、与SPS、SS-S活性呈先下降后上升趋势,AI与NI在贮藏过程中出现明显峰值,且1-MCP处理可以显著提高这一峰值。
[Key word]
[Abstract]
Through using Aksu Fuji apple as the test material, and analyzing the sucrose, glucose, fructose and sorbitol contents as along with sucrose phosphate synthase (SPS), sucrose synthase (SS-S, SS-C), acid invertase (AI), neutral invertase (NI) and sorbitol dehydrogenase (SDH) activity, this study examined the changes of sugar metabolism and related enzyme activities of these apples harvested at different stages. The effects of 1-MCP treatment on them were analyzed during storage (0~150 days). The obtained results showed that 1-MCP treatment during postharvest storage inhibited the increase of sucrose, glucose, fructose and sorbitol contents in Fuji apple fruit during storage, and exerted different effects on the enzymatic activities in the apples harvested at different stages. The sucrose and sorbitol contents and SDH activity in fruit decreased, whilst the contents of fructose and glucose and activities of SPS and SS-S decreased first and then increased. AI and NI showed significant peak values during storage, and 1-MCP treatment significantly increased these peaks.
[中图分类号]
[基金项目]
“十二五”农村领域国家科技计划项目(2011BAD27B01-02)