樟树被证明具有许多生物活性，包括抗真菌、抗氧化、抗菌、抗过敏、抗炎活性。然而，很少有研究报道樟树果实多糖（CCFP）。本研究的目的首先从樟树果实中分离出CCFP，然后通过体外实验评估其对RAW264.7巨噬细胞免疫调节活性。结果表明，CCFP可显著提高RAW 264.7细胞内一氧化氮（NO）的浓度和细胞因子如前列腺素E2（PGE2）和肿瘤坏死因子（TNF-α）的分泌，在给药浓度为100 μg/mL时，NO、TNF-α和PGE2的分泌量达到最大，分别为53.53±4.25 μM、1008.32±35.23 pg/mL和434.56±25.23 pg/mL。反转录聚合酶链式反应（RT-PCR）分析结果表明，CCFP也能够提高诱导型一氧化氮合酶（iNOS）、环氧化酶-2（COX-2）和TNF-α的表达。进一步的分析表明，CCFP通过与Toll样受体4（TLR4）蛋白结合，迅速激活细胞外信号调节激酶（ERK）、转录激活因子激活蛋白-1（AP-1）和核因子（NF）-κB。这些结果表明，CCFP可以提高免疫力，在功能食品的开发中，可以作为一个潜在的免疫调节剂。

Cinamomum camphora is proved to possess a lot of biological activities, including antifungal, antioxidant, antibacterial, anti-allergic, and anti-inflammatory activities. However, little research has been reported on the C. camphora fruit polysaccharides (CCFP). Therefore the objective of this study was to isolate polysaccharide from C. camphora and evaluate its immunomodulatory activities on Macrophage RAW264.7. The results indicated that CCFP significantly promoted the production of intracellular nitric oxide (NO) and cytokines such as prostaglandin E2 (PGE2) and tumor necrosis factor-α (TNF-α) in RAW 264.7 cells. When the dosage of CCFP was 100 μg/mL, the secretion of NO, TNF-α and PGE2 reached to the maximum values of 53.53 ± 4.25 mu M, 1008.32 ± 35.23 pg/mL and 434.56 ± 25.23 pg/mL, respectively. The result of RT-PCR analysis indicated that CCFP also enhanced inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and TNF-α expression. Further analyses demonstrated that CCFP rapidly activated the extracellular signal-regulated kinase (ERK) and enhanced the transcriptional activities of activator protein-1 (AP-1) and nuclear factor (NF)-κB via toll-like receptor 4 (TLR4). Taken together, these results suggest that CCFP can improve immunity, and could be explored as a potential immunomodulatory agent in functional food.

江西省重点研发计划项目（20171BBH80017）；江西省科技计划项目（20151BBF60024）；江西省科学院博士人才引进项目（2016-YYB-07）