紫檀芪对Ⅱ型糖尿病大鼠血脂的改善及对PPARγ的调控作用

The Improvement of Blood Lipid in Type Ⅱ Diabetic Rats by Pterostilbene and its Modulation of PPARγ Expression

摘要:

本文探讨紫檀芪对Ⅱ型糖尿病大鼠血脂的影响及对脂肪组织中过氧化物酶体增殖物激活受体γ (PPARγ)的调节。将实验大鼠分为正常对照组、Ⅱ型糖尿病组、紫檀芪低剂量组、紫檀芪中剂量组、紫檀芪高剂量组。全自动分析仪检测血脂各项，WST-1法检测血清中SOD活性，TBA法检测MDA含量，荧光定量PCR和western blot检测脂肪组织中PPARγmRNA和蛋白的表达。结果显示：与正常对照组相比，Ⅱ型糖尿病组大鼠TG、TC、LDL-C水平升高，HDL-C水平降低，SOD活性降低，MDA水平升高（p<0.05）。与Ⅱ型糖尿病组相比，紫檀芪低、中、高剂量组TG降低；紫檀芪低、中剂量组TC、LDL-C降低，SOD升高，MDA下降（p<0.05）。与正常对照组相比，Ⅱ型糖尿病大鼠脂肪组织中PPARγmRNA和蛋白表达显著降低；紫檀芪低、中、高剂量组大鼠脂肪组织中PPARγmRNA蛋白表达量较Ⅱ型糖尿病组显著增加。因此，紫檀芪可以改善Ⅱ型糖尿病的血脂紊乱并调节氧化应激水平，其机制可能与调节PPARγ有关。

Abstract:

The effects of pterostilbene (PTE) on blood lipid and PPAR γ expression of adipose tissues in type Ⅱ diabetic rats were investigated. Rats were randomly divided into five groups: normal control group, type Ⅱ diabetes mellitus (T2DM) group, PTE low dose group, PTE middle dose group and PTE high dose group. Blood lipid, the activity of SOD and the level of MDA were examined, and PPARγ mRNA and protein expression in adipose tissue were evaluated. As compared to normal rats, the levels of TG, TC, LDL-C and MDA in the T2DM group increased, while the level HDL-C and SOD decreased. The level of TG in PTE (low, middle and high) groups and TC, LDL-C and MDA in PTE (low and middle) groups were lower than those in the T2DM groups , while the activity of SOD in PTE (low and middle) groups was higher than the T2DM group (p < 0.05). As compared with normal rats, the mRNA and protein expression of PPARγ significantly decreased. Moreover, the mRNA and protein expression of PPARγ significantly increased in PTE rats as compared to the T2DM rats. Therefore, PTE can improve the the level blood lipid and mediate the state of oxidative stress, and the underlying mechanism might be associated with the regulation of PPARγ expression.