Abstract:A simple and quick analysis method was established for the determination of streptomycin, dihydrostreptomycin and kanamycinresidues in fish products by solid-phase extraction coupled with liquid chromatography-tandem mass spectrometry. The sample was extracted with sodium heptanesulfonate and phosphate buffer, and cleaned with C18, The separation was carried out on a ACQUITYUPLC BEH HILIC C18 column with a gradient elution using acetonitrile and 0.1% formic acid, The mass spectrometer was operated in the positive ion mode using selected reaction monitoring for the qualitative and quantitative analysis. The external standard calibration curve was used for the quantification. The linear ranges were from 5.0 to 100 μg/kg with a good linear relationship (R2>0.9992). The detection limit of this method was 2.5 μg/kg, and the limit of quantification was 5.0 μg/kg. The relative standard deviations ranged from 1.16%~7.35% (n=6) and the average recovery rates were from77.4%~107.3%. The method was suitable for the identification and quantification of streptomycin, dihydrostreptomycin and kanamycin residues in fish products due to high reproducibility and selectivity.
