可过滤液体饮料中微生物学检验滤膜法和平板计数法的等效性比较分析

Comparative Analysis of the Equivalence of Filter Membrane Method and Plate Counting Method in Microbiological Examination of Filterable Liquid Beverages

摘要:

比较分析滤膜法和平板计数法在检验可过滤饮料中菌落总数、霉菌和酵母计数以及大肠菌群计数微生物指标是否具有等效性。通过预实验结果，采集可过滤液体饮料样品分别加入定量的枯草芽孢杆菌、白色念珠菌、黑曲霉以及大肠杆菌制备成样品，分别采用滤膜法与平板计数法分别检测菌落总数、霉菌计数、酵母计数以及大肠菌群计数，参照采用ISO 17994:2014《水质-用两种定量方法对比微生物回收率的基本要求》对滤膜法和平板计数法的检测结果，然后进行数据比较分析，以验证两种方法在可过滤液体饮料微生物学检验中的是否具有等效性。结果表明当取样量为100时，滤膜法和平板计数法在检测可过滤液体饮料微生物学检验中具有等效性。滤膜法与平板计数法应用于可过滤饮料中微生物学指标的检验可考虑相互取代。

Abstract:

The aim of this study was to investigate whether there is equivalence between filter membrane method and plate counting method in examination of microbiological indexes of filterable beverage which include total numbers of colony and the count of mold, yeast and coliform. Through pre-experimental results, 100 copies of filterable liquid beverage were collected and then added with quantitative bacterial fluid for examination of total numbers of colony and the count of mold, yeast and coliform by using filter membrane method and plate counting method respectively. After that, comparative analysis on results of the two methods was conducted based on ISO 17994:2014 "Water quality- Requirements for the comparison of the relative recovery of microorganisms by two quantitative methods ", so that the equivalence of the two methods in microbiological examination of filterable liquid beverage could be verified. The results indicated that, filter membrane method and plate counting method have equivalence in microbiological examination of filterable liquid beverage when its sampling quantity reaches 100. Thus, the two methods are alternative when applied to the examination of microbiological indexes of filterable liquid beverage.