狭叶荨麻醇提物的体外抗氧化及抗炎活性

Evaluation on the Antioxidant and Anti-inflammatory Activities of the Ethanol Extracts from Urtica Angustifolia in Vitro

摘要:

为了开发利用狭叶荨麻资源，本研究以狭叶荨麻为原料，对其醇提物的体外抗氧化及抗炎活性进行了研究。在单因素试验的基础上，采用响应面法对提取条件进行了优化，得到最佳提取条件为提取时间114 min、料液比1:15、乙醇浓度为64%，所得醇提物得率（10.00±0.26）%，该狭叶荨麻醇提物中活性物质含量总酚（160.77±0.01）mg/g、总黄酮（366.85±0.05）mg/g、原花青素（7.81±0.01）mg/g、皂苷（516.76±0.04）mg/g、生物碱（2.01±0.02）mg/g。在优化条件下进行了提取，分别采用抗坏血酸（Vc）、双氯芬酸钠作抗氧化、抗炎阳性对照，对所得狭叶荨麻醇提物进行总还原力、清除DPPH•、清除•OH及抑制透明质酸酶、抑制白蛋白变性活性评价。该狭叶荨麻醇提物总还原力高于Vc，且量效关系比Vc更明显（p<0.001），EC50为0.04 mg/mL；清除DPPH•、清除•OH活性比Vc好，IC50分别是0.02 mg/mL、1.87 mg/mL；抑制透明质酸酶、抑制白蛋白变性活性与双氯芬酸钠相近，IC50分别是2.43 mg/mL，0.31 mg/mL。

Abstract:

Urtica Angustifolia was used as raw material to evaluate its antioxidant and anti-inflammatory activities in this research in order to develop Urtica Angustifolia resources. Based on the single factor tests, the optimum extraction conditions were determined by response surface methodology. The results showed that the optimum extraction conditions were as follows: extraction time 114 min, solid-liquid 1:15, ethanol concentration 64%. Under the optimal conditions, the ethanol extracts yield was (10.00±0.26)%, and in which the content of active substances total phenols, total flavonoids, procyanidins, saponins, alkaloids were (160.77±0.01) mg/g, (366.85±0.05) mg/g, (7.81±0.01) mg/g, (516.76±0.04) mg/g, (2.01±0.02) mg/g respectively. Meanwhile, Vitamin C and diclofenac sodium were used as antioxidant and anti-inflammatory positive control respectively to evaluate the total reducing power, capacities of scavenging DPPH•, scavenging •OH and activities of inhibiting hyaluronidase and albumin denaturation. The reducing power of the Urtica Angustifolia ethanol extract was higher than Vc (p<0.001) and its EC50 was 0.04 mg/mL. The DPPH? and ?OH scavenging capacities were higher than Vc, and the IC50 on DPPH• and •OH scavenging were 0.02 mg/mL, 1.87 mg/mL respectively. The inhibition of the Urtica Angustifolia ethanol extracts on hyaluronidase and inhibited albumin denaturation activity were similar to diclofenac sodium, and their IC50 values were 2.43 mg/mL and 0.31 mg/mL respectively.