GC-MS分析使用不同提取工艺获得的沙枣花挥发性成分

GC-MS Analysis of the Volatile Compounds Derived from Elaeagnus Angustifolia under Different Extraction Conditions

摘要:

这项研究采用水蒸气蒸馏法、有机溶剂浸提法、真空泵抽取吸附采集法、固相微萃取法以及超临界CO2萃取法对新疆沙枣花挥发油进行提取，并探讨不同提取方法对沙枣花挥发性化合物成分的影响。通过气相色谱-质谱（GC-MS）联用技术对沙枣花挥发油的化学成分进行分析鉴定，用峰面积归一法确定各组分的相对含量。共分离鉴定出139种化合物，其中共检测出烃类22种（占21.3%）、醛类10种（占1.38%）、酯类47种（占62.18%）、酮类7种（占0.6%）、醇类21种（占9.52%）、酸类14种（占1.86%）、苯环类7种（占1.66%）、酚类5种（占1.14%）、其它物质6种（占0.33%）。其中共有成分4种，分别是2,3-丁二醇、苯乙酸乙酯、苯乙醇、反式-肉桂酸乙酯。

Abstract:

In this study, steam distillation, organic solvent extraction, vacuum pump extraction and adsorption, solid phase microextraction (SPME), and supercritical CO2 extraction were used to extract the volatile oil of E. angustifolia flower grown in Xinjiang, and examined the effects of these different extraction methods on of the profile of the obtained volatile compounds. Gas chromatography-mass spectrometry (GC-MS) was used to analyze and identify the chemical constituents of volatile oil from the E. angustifolia flowers, with the relative amount of each component being determined using the peak area normalization method. A total of 139 compounds were isolated and identified, including 22 hydrocarbon (21.3%), 10 aldehydes (1.38%), 47 esters (62.18%), 7 ketones (0.6%), 21 alcohols (9.52%), 14 acids (1.86%), 7 benzenes (1.66%), 5 phenols (1.14%), and 6 other species (0.33%). Among which, four kinds of components, namely2,3- butanediol, ethyl phenylacetate, phenethyl alcohol and ethyl trans-cinnamate were found.