采用本体聚合法，以盐酸四环素为模板分子，甲基丙烯酸为功能单体，乙二醇二甲基丙烯酸酯为交联剂，偶氮二异丁腈为引发剂，合成了针对四环素类抗生素具有选择性的分子印迹聚合物。并以此材料作为固相吸附剂与高效液相色谱联用，建立了固相萃取-高效液相色谱技术检测鸡肉中的3种四环素类抗生素痕量残留的方法。实验结果表明，该材料具有较强的吸附性和特异识别性。在0.1 mg/L~1.0 mg/L范围内3种四环素类药物的线性关系良好（R2>0.99），土霉素、四环素、强力霉素的最低检出限分别为0.13 μg/L、0.12 μg/L、0.14 μg/L，5次重复实验的精密度分别为1.27%、1.31%、1.94%。在鸡肉中添加3个浓度（50 μg/kg、100 μg/kg、200 μg/kg）的四环素类抗生素，回收率分别为88.33%~94.95%、80.94%~88.02%、87.69%~93.08%，RSD分别为2.08%~3.63%、1.46%~2.37%、1.04%~3.03%。这项研究的结果表明目前建立的方法可以用于鸡肉中四环素类抗生素的残留检测。

A selective molecularly imprinted polymer for separating tetracycline antibiotics was synthesized via bulk polymerization using tetracycline hydrochloride as the template, methacrylic acid as a functional monomer, ethylene glycol dimethacrylate as a cross-linker and azobisisobutyronitrile as an initiator. This synthesized material was used as the solid phase adsorbent that was coupled with high performance liquid chromatography (HPLC) to establish a solid phase extraction-HPLC system for the determination of trace residues of three tetracycline antibiotics in chicken meat. The obtained results showed that the material has high adsorption capacity and specific recognition. A good linearity of the three tetracycline antibiotics in the range of 0.1-1.0 mg/L (R2 > 0.99) was detected, with the minimum detection limits of oxytetracycline, tetracycline, doxycycline as 0.13 μg/L, 0.12 μg/L, 0.14 μg/L, respectively, and relative standard deviation (RSD) values of the five replicates as 1.27%, 1.31%, 1.94%, respectively. Whentetracycline antibiotics were added to chicken at three different concentrations (50 μg/kg, 100 μg/kg and 200 μg/kg, respectively), the recoveries were 88.33%~94.95%, 80.94%~88.02%, and 87.69%~93.08%, respectively, with corresponding RSD values being 2.08%~3.63%, 1.46%~2.37%, and 1.04%~3.03%, respectively. The results of this study show that the currently established method can be used for the detection of tetracycline residues in chicken meat.

国家自然科学基金项目（31301470）；山东省优秀中青年科学家基金项目（BS2012SW018）