代代花化学成份的分离鉴定和抗氧化活性研究

Isolation and Identification of Compounds with Antioxidant Activity from Citrus aurantium L. var.amara Engl.

摘要:

为了探讨代代花化学成分的抗氧化活性，本文以代代花乙醇提取物为原料，采用硅胶柱、葡聚糖凝胶柱和聚酰胺柱等传统柱层析技术，结合高效液相色谱和核磁共振波谱等现代技术手段，从代代花氯仿相和乙酸乙酯相中分离纯化出3个化合物，分别鉴定为高圣草素、佛手酚和南酸枣苷，三者均为首次从代代花中分离得到。利用ABTS、DPPH、FRAP、总还原力四种体外抗氧化检测体系对三种化合物进行了抗氧化活性研究。总还原力测定实验中，佛手酚显示了良好的抗氧化能力，且效果优于高圣草素和南酸枣苷。ABTS自由基清除实验中，在最高浓度800 μg/mL时，佛手酚和高圣草素的清除率分别为100%和95%，二者清除效果接近或等于阳性对照。DPPH自由基清除实验中，佛手酚的抗氧化效果最佳，其在最高浓度时的清除率达到79%。铁离子还原实验中，佛手酚和高圣草素在800 μg/mL时的FRAP值分别为1.5585和1.1239，二者的铁离子还原能力比较接近于阳性对照的2.1682。

Abstract:

To investigate the components with antioxidant activity from Citrus aurantium L. var.amara Engl., the Citrus aurantium L. var.amara Engl. alcohol extract was purified by several traditional column chromatography technologies such as silica gel column, dextran gel column and polyamide column, combined with HPLC,NMR and other modern technologies. Three compounds were separated from chloroform and ethyl acetate extract and identified as homoeriodictyol, bergaptol and choerospondin. All of them were first isolated from Citrus aurantium L. var.amara Engl. Antioxidant activities were examined by four external evaluation systems(Reductive power measurement,ABTS, DPPH and FRAP). Bergaptol showed excellent antioxidant ability based on reductive power measurement, which is better than that of homoeriodictyol and choerospondin. By ABTS method the radical-scavenging clearance of bergaptol and homoeriodictyol were 100% and 95%, respectively, at the concentration of 800 μg/mL, which was equal or close to positive control. DPPH radical scavenging ability measurement revealed that the antioxidant effect of bergaptol was the best, and the clearance reached 79% at the highest concentration. FRAP assay indicated that the FRAP value of bergaptol and homoeriodictyol were 1.5585 and 1.1239, respectively, close to 2.1682 of positive control.