野葛根酸性多糖的结构鉴定及免疫活性

Identification and Immunomodulatory Activity of a Acidic Polysaccharide from the Pueraria lobate (Willd.) Ohwi Root

摘要:

本文采用离子交换层析结合凝胶渗透色谱技术从野葛根的热水浸提液中分离纯化得到一种新型酸性多糖，鉴定了该多糖的结构特征，并初步探究了其免疫调节活性。结果显示，该酸性多糖是一种分子量约为12300 u且具有一定支链结构的葡聚糖，不具有三螺旋空间结构，其糖苷键类型为α-（1→4）和α-（1→4，6）。以小鼠巨噬细胞RAW264.7为实验对象，研究发现该酸性多糖能有效地刺激细胞分泌细胞因子NO、TNF-α和IL-6，进而体现出较强的免疫调节活性。

Abstract:

A novel acidic polysaccharide was isolated and purified from the hot-water extract of Pueraria lobate (Willd.) Ohwi root, using a sequential ion exchange chromatography and gel-filtration chromatography. The structural features of this acidic polysaccharide were characterized, and its immunomodulatory activity investigated. This acidic polysaccharide is a homogeneous glucan with branched chains, with an average molecular weight of 12300 u, which has no three spiral space conformation. The main linkage types of this polysacchide were α-(1→4) and α-(1→4, 6). Using RAW264.7 cells as the model, this acidic polysaccharide has been confirmed to exhibit an immunomodulatory activity through promoting the secretion of NO, IL-6, and TNF-α.