Abstract:A simple, green and efficient method for the determination of polydatin and resveratrol in Polygonum Cuspidatum was established by the ultrasound-assisted ionic liquid / anionic surfactant aqueous two-phase extraction coupled with high performance liquid chromatography. In this study, the water-soluble ionic liquid was used as the extraction solvent and the anionic surfactant was used as the salt phase, and the target analyte was extracted into the sample solution with the aid of ultrasound. The experimental results showed that the optimum extraction conditions: 400 μL of [C4MIM][BF4] was used as extraction solvent, 0.16 g of sodium dodecylbenzenesulfonate (SDBS) was used as salt phase, the pH of sample solution was 7.0, and the analyte was extracted for 20 min at 160 W. All the compounds had good linearity in the linear range (r>0.9995), and the RSDs of intra-day and inter-day precision were lower than 3.27% and 6.31%, respectively. The limits of detection (LOD) and quantification (LOQ) were 2.8~12.3 and 9.4~41.1 ng/mL, respectively, and the spiked recoveries were between 86.88% and 94.13%. In this study, ionic liquid was used as an extraction agent (instead of traditional organic solvent), which combined extraction, concentration, purification and separation. The proposed method has the advantage of simple experiment operation, less solvent consumption and short extraction time, which could be widely used in the extraction and analysis of active components in traditional Chinese medicine in the future.
