超高效液相色谱法测定甘蓝和橙子中的阿维菌素残留

Determination of Abamectin Residues in Cabbage and Orange by Ultra Performance Liquid Chromatography

摘要:

本文研究建立了甘蓝和橙子中阿维菌素农药残留的超高效液相色谱紫外（UPLC-UV）检测方法。样品经乙腈提取，混合使用乙二胺-N-丙基硅烷（PSA）和C18基质分散净化剂进行净化，采用ACQUITY UPLC BEH C18色谱柱（2.1×100 mm，1.7 μm），以乙腈:水（V/V=70:30）为流动相，流速0.25 mL/min，柱温：35 ℃，紫外检测波长：245 nm。阿维菌素在甘蓝和橙子样品中存在程度不同的基质效应，故采用空白基质匹配的校准曲线外标法定量。结果表明，阿维菌素在0.25~10 mg/L范围内具有良好的线性关系，相关系数均大于0.9999。在0.05~0.5 mg/kg范围内，平均添加回收率为83.0%~100.2%，相对标准偏差为1.17%~1.30%。阿维菌素在甘蓝和橙子中的检出限（LOD）分别为0.0109和0.0160 mg/kg。该方法操作简单、准确、灵敏，可用于甘蓝和橙子中阿维菌素的分析。

Abstract:

A determination method for abamectin residues in cabbage and orange was established in this study by using ultra high performance liquid chromatography- ultraviolet (UPLC-UV). Samples were extracted with acetonitrile, and mixed with ethylenediamine-N-propylsilane (PSA) and C18 matrix dispersion scavenger for purification by using a ACQUITY UPLC BEH C18 column (2.1×100 mm, 1.7 μm). The column temperature was set as 35 ℃, the mobile phase was composed of acetonitrile-water (70:30, V/V) with a flow rate of 0.25 mL/min, and the ultraviolet detection wavelength was set as 245 nm. Abamectin had different matrix effects on cabbage and orange samples and therefore, external standard of the blank matrix-matched calibration curves was used for quantification. The results showed that abamectin had good linear relationship in the range of 0.25~10 mg/L, and the correlation coefficients were all more than 0.9999. In the range of 0.05~0.5 mg/kg, the average addition recovery was 83.0%~100.2%, and the relative standard deviation (RSD) was 1.17%~1.30%. The limits of detection (LOD) of abamectin in cabbage and orange (S/N=3) were 0.0109 mg/kg and 0.0160 mg/kg, respectively. The proposed method was simple, accurate and sensitive, which could be used for the analysis of abamectin in cabbage and orange.