牛樟芝对四氯化碳致大鼠慢性肝损伤的保护作用

Protective Effects of Antrodia Cinnamomea on Chronic Liver Injury Induced by Carbon Tetrachloride in Rats

摘要:

本文研究了牛樟芝对四氯化碳（CCl4）致大鼠慢性肝损伤的保护作用。将雄性SD大鼠随机分为6组：正常组、模型组、牛樟芝低（30 mg/kg）、中（60 mg/kg）、高（120 mg/kg）剂量组及秋水仙碱阳性对照组（0.1 mg/kg）。除正常组外，均腹腔注射50% CCl4橄榄油溶液，1 mL/kg，2次/周，连续9周，建立慢性肝损伤模型，造模的同时，牛樟芝组及阳性组灌胃给相应药物，正常组和模型组灌胃给蒸馏水，每日1次，连续9周。处死大鼠，检测血清中ALT、AST活性；肝组织中SOD、MDA、GSH-Px及GSH；计算脏器指数；HE染色观察肝组织的病理变化。结果表明，与模型组相比，牛樟芝可明显改善肝脏病理变化，可显著降低CCl4所致慢性肝损伤大鼠血清中ALT、AST活性（p<0.01），同时高剂量组显著升高肝脏组织匀浆上清中SOD、GSH-Px活性、GSH含量，并显著降低MDA含量（p<0.05，p<0.01）。因此牛樟芝对CCl4所致大鼠慢性肝损伤有明显的保护作用。

Abstract:

The protective effects of Antrodia cinnamomea(AC) on the chronic liver injury induced by carbon tetrachloride (CCl4) in rats were investigated in this study. First of all, male Sprague-Dawley (SD) rats were randomly divided into six groups: normal group, model group, low-dose of AC group (30 mg/kg), middle-dose of AC group (60 mg/kg), high-dose of AC group (120 mg/kg) and colchicine positive control group (0.1 mg/kg). Subsequently, in order to establish the model of chronic liver injury, all groups, except for the normal group, were injected intraperitoneally (i.p.) with 50% CCl4 (CCl4: olive oil= 1:1; V/V) at 1ml/kg body weight twice a week for 9 weeks. At the same time, AC groups and positive group were treated with the corresponding drugs once daily for 9 weeks. Correspondingly, the normal group and model group were gavaged with equal volume of distilled water once daily for 9 weeks. The rats were sacrificed and the activity of alanine amiotransferase (ALT) and aspartate aminotransferase (AST) in the serum were measured.; the activity of the superoxide dismutase (SOD), malondialdehyde (MDA), glutathione peroxidase (GSH-Px) and glutathione (GSH) in the liver tissue were measured. The indexes of the organ were calculated. The pathological changes of liver tissue in rats were observed by HE staining. The results showed that, compared with the model group, AC could significantly improve the pathological changes of the liver, and could significantly reduce the activity of ALT and AST in the serum of rats with chronic liver injury induced by CCl4 (p<0.01). Meanwhile , the activity of SOD, GSH-Px and GSH in the liver homogenate supernatant were significantly increased in high dose group, but the level of MDA was sharply declined in high dose group (p<0.05, p<0.01). Therefore, it was concluded that Antrodia cinnamomea had an obvious protective effect on chronic liver injury induced by CCl4 in rats.