Abstract:Hydrolyzed Vegetable Protein (HVP) was a typical adulterated compound that was used as an inexpensive protein by criminals to increase the nitrogen content in milk. Different concentrations of HVP adulterated samples were prepared in this study and were scanned directly with the near-infrared spectrometer (NIRs). Samples pretreated with trichloroacetic acid were analyzed by Transmission Analyzer, and the quantitative analysis models were established by TQ software to compare the difference between the two methods. Results showed that, the optimized quantitative analysis model by Fiber Optic Module was established with 10 principle components factors through preprocessing method of first derivative & Norris derivative filter in the spectral region of 8000~5000 cm-1. The root mean square error of cross (RMSEC), root mean square error of prediction (RMSEP) and correlation coefficient (R) of the model were 0.224, 0.214 and 0.98414 respectively with a recovery of 93.2479%., The optimized quantitative analysis model by transmission analyzer was established with 10 principle components factors through preprocessing method of first derivative & Norris Derivative Filter in the spectral region of 10000~7000 cm-1. RMSEC, RMSEP and R of the model were 0.175, 0.138 and 0.99036 respectively with a recovery of 98.7351%. The quantitative analysis model pretreated with tricholoroacetic acid was accurate and stable, which could be used in rapid quantitative analysis of HVP in milk and provide reference for milk quality control.
