恩诺沙星分子印迹聚合物膜的制备及吸附性能研究

Preparation and Adsorption Properties of Enrofloxacin MolecularImprinted Polymer Film

摘要:

采用表面印迹法，以恩诺沙星为模板，甲基丙烯酸（MAA）为功能单体，乙二醇二甲基丙烯酸酯（EDMA）为交联剂，在聚苯乙烯酶标板表面直接合成恩诺沙星分子印迹聚合物膜。通过傅立叶红外光谱分析、电镜扫描、吸附平衡结合实验、Scatchard方程分析及吸附动力学实验对恩诺沙星印迹聚合物膜进行性能表征。合成的分子印迹聚合物膜具有很好的印迹效果，对恩诺沙星有较高的特异性吸附，且传质速率快，由Scatchard方程分析可知，聚合物膜中含有两类吸附位点，其中高亲和力位点的平衡解离常数（Kd）为19.49 μg/mL，饱和吸附容量（Qmax）为12.98 μg/mL，低亲和力位点的Kd为277.78 μg/mL，Qmax为98.14 μg/mL，吸附位点的异质性并不会影响聚合物膜应用于竞争性免疫吸附分析。通过该方法合成的恩诺沙星特异性识别聚合物膜可以作为仿生抗体，应用于竞争性免疫吸附分析检测恩诺沙星在食品中的残留。

Abstract:

The enrofloxacin molecular imprinted polymer film was directly synthesized on the surface of polystyrene microtiter plate by surface imprinting technique using the enrofloxacin (ENRO) as template, methacrylic acid (MAA) as functional monomer and ethyleneglycol dimethacrylate (EDMA) as cross-linker. The enrofloxacin molecular imprinted polymer film was characterizedby FT-IR, scanning electron microscope, adsorption-equilibrium experiments, scatchard analysis and adsorption kinetic experiments. The results showed that the imprinted film exhibited good imprinting effect, high specific adsorption ability and fast mass transfer rate. Scatchard analysis indicated that the imprinted film contained two types of binding sites with specific binding properties. The dissociation constant (Kd) and maximum binding capacity (Qmax) of the two linear curves were 19.49μg/mL, 12.98 μg/mL for the higher affinity sites and 277.78 μg/mL, 98.14 μg/mL for the lower affinity sites, respectively, and the heterogeneous of MIP could be ignored in MIAs. The enrofloxacin- specific polymer film synthesized by the proposed method could be used as a biomimetic antibody to determine the enrofloxacin residues in food by competitive immunosorbent assay.