Abstract:The effect of extraction temperature on the structure of Lentinan polysaccharide was investigated three kinds of Lentinan polysaccharide samples, including LEP-A, LEP-B and LEP-C, were obtained from Lentinus edodes by hot water extraction method. The extraction conditions were as follows: the ratio of material to liquid was 1:20 and extraction times for 2 with different extraction temperature changing from 100℃ to 140℃. The content of polysaccharide significantly increased with the increase of extraction temperature. High performance liquid chromatography (HPLC) analysis showed that the monosaccharide composition of three crude polysaccharides contained only glucose. Analysis of IR spectrum proved that three kinds of polysaccharides contained the stretching vibration of -OH at 3600- 3000 cm-1, the stretching vibration of -CH at 3000 -2800 cm-1, and the stretching vibration of carboxyl at 1430-1200 cm-1. All of them were the characteristic IR absorption peaks of polysaccharides. In combination with other characteristic absorption peaks, the polysaccharide was confirmed as a pyran polysaccharide. LEP-A, LEP-B and LEP-C immunoreactivity analysis showed that the three kinds of Lentinan polysaccharide samples could improve the phagocytosis of macrophages and stimulate the proliferation rate of T lymphocytes and B lymphocytes. Also, the activities of polysaccharide obtained at low extraction temperature such as 100℃ and 119℃ were higher than that at 140℃.
