双酚A（BPA）的内分泌干扰作用以及对人体产生的不良影响，使双酚S（BPS）作为替代品在食品包装材料中应用更加广泛。BPS在大规模应用之前，并没有对其安全性进行充分研究。本文以3T3-L1前脂肪细胞为体外模型，探究BPS对3T3-L1前细胞分化的影响及作用途径，评价BPS安全性的同时，也为肥胖等慢性代谢疾病的预防提供参考。研究结果表明：BPS能促进3T3-L1前脂肪细胞生长，当浓度超过400 μmol/L时，才能显著抑制细胞的增殖；BPS能显著诱导分化的3T3-L1细胞内脂质的积累，并呈现非剂量依赖关系；与模型组相比，BPA上调PPARγ、C/EBR和aP2基因的表达，而BPS只作用PPARγ基因的过表达，BPS和BPA都能使GLUT4基因的表达显著下降（p<0.01）。总之BPS和BPA作用脂肪细胞分化的途径不完全一样，可能是在多种转录因子的共同作用下完成的。

Bisphenol A (BPA) is restricted for its endocrine disruption and adverse health effects on the human body, so that bisphenol S (BPS) is widely used as a substitute n a variety of food packaging materials. The security of BPS has not been fully studied before its large-scale application.. In this study, 3T3-L1 preadipocytes were used as an in vitro model to investigate the effect of BPS on the differentiation of 3T3-L1 preadipocytes and its mechanism, to evaluate the safety of BPS and provide reference for the prevention of chronic metabolic diseases such as obesity. The results showed that BPS could promote the growth of 3T3-L1 preadipocytes and could significantly inhibit the proliferation of cells when the concentration was over 400 μmol/L. BPS could significantly induce the accumulation of lipid in the differentiated 3T3-L1 cells which showed a non-dose-dependent relationship. Compared with the model group, BPA up-regulated the expressions of PPARγ, C/EBR and aP2 genes, whereas BPS only seems to up-regulate the expression of PPARγ. In addition, BPS and BPA could significantly decrease the expression of GLUT4 gene (p<0.01). In conclusion, the pathways of BPS and BPA acting on adipocytes differentiation were not exactly the same, which might be accomplished under the combined action of various transcription factors.

国家自然科学基金资助项目（31501569）；江苏大学高级专业人才专项（13JDG037）