葛根总黄酮与葛根素提取工艺优化及增强结肠炎大鼠抗氧化能力

Optimization of Extraction Technology of Total Flavonoids and Puerarin in Rdix Puerariae and their Enhanced Antioxidant Capacity in Colitis Rats

摘要:

在传统乙醇热回流法提取葛根黄酮的基础上，采用二次正交旋转组合设计优化同步提取葛根总黄酮和葛根素工艺。由葛根总黄酮得率(y1)和葛根素得率(y2)与提取温度(x1)、回流时间(x2)、乙醇浓度(x3)、料液比(x4)等四个试验因子分别建立回归模型。通过回归模型分析发现，葛根黄酮提取中最显著的影响因素为提取温度(x1)和乙醇浓度(x3)，且得到的最佳提取条件为提取温度85 ℃、回流时间55 min、乙醇浓度30%、料液比1:17.5 (g:mL)，在此条件下葛根总黄酮提取率可达到41.07 mg/g，葛根素提取率可达到16.50 mg/g，优化所得的提取工艺稳定可行。在结肠炎模型下葛根提取物相较于纯品葛根素而言，能更好地提高大鼠血清CAT、SOD、GSH-px水平，即能更好地提高机体抗氧化能力。

Abstract:

Based on the traditional ethanol heat reflux method to extract puerarin and flavone, the quadratic-orthogonal-rotation -combination design was used to optimize the extraction of total flavonoids and puerarin from Rdix Puerariae. Regression models were established for the yield of total flavonoid (y1) and the yield of puerarin (y2) with the other four test factors (including extraction temperature (x1), reflux time (x2), ethanol concentration (x3) and ratio of material to liquid (x4), respectively. The regression model showed that, the most important two factors that affected the total flavonoids yield and puerarin yield from Radix Puerariae were: extraction temperature (x1) and ethanol concentration (x3). The optimal technical conditions were: extracting at 85 ℃ for 55 min, reflux time 55 min, ethanol concentration of 30%, solid-to-liquid ratio of 1:17.5. Under these conditions, the extraction rate of total flavone from Radix Puerariae was 41.07 mg/g, the extraction of puerarin rate could be 16.50 mg/g, and the optimized extraction technology of Radix Puerariae was stable and feasible. The results showed that the Rdix Puerariae extractive had a better effective in recovering serum catalase (CAT), Cu/Zn superoxide dismutase (SOD), and phospholipid hydroperoxide glutathione peroxidase (GSH-px) levels as well as antioxidant capacity in colitis rats as compared with pure puerarin.