探讨猫豆乙醇提取物(MPEE)对胰岛素抵抗3T3-L1脂肪细胞糖脂代谢的影响。用含高浓度葡萄糖和高浓度胰岛素的DMEM培养基制备胰岛素抵抗3T3-L1脂肪细胞模型。模型细胞经不同浓度MPEE处理24 h后检测培养液中的葡萄糖消耗量、游离脂肪酸(FFA)、甘油、三酯甘油(TG)及肿瘤坏死因子(TNF-α)和白介素6(IL-6)水平。实时定量PCR(qRT-PCR)检测细胞中葡萄糖转运蛋白(GLUT-4)、激素敏感脂肪酶(HSL)和炎性介质[TNF-α、IL-6、单核细胞趋化蛋白-1(MCP-1)、C反应蛋白(CRP)]的mRNA表达。MPEE可促胰岛素抵抗模型细胞对葡萄糖的消耗，降低细胞中TG含量，减少FFA和甘油溢出。同时，MPEE还能抑制模型细胞TNF-α和IL-6分泌，及炎性介质(TNF-α、IL-6、MCP-1、CRP)的mRNA表达。此外，MPEE能上调细胞中GLUT-4的表达，并降低HSL的mRNA表达。结果显示，MPEE可有效刺激3T3-L1脂肪细胞消耗葡萄糖，促TG分解，减少FFA和甘油的溢出。调控细胞糖、脂代谢来改善胰岛素抵抗，并降低胰岛素抵抗发生时的炎症水平。

To investigate the effect of Mucuna pruriens (Linn) ethanol extracts (MPEE) on glucose and lipid metabolism in insulin resistance 3T3-L1 adipocytes. The insulin resistance 3T3-L1 adipocyte model was induced by DMEM medium containing high concentration of glucose and insulin. The levels of glucose consumption, free fatty acid (FFA), glycerol, cellular triglyceride(TG), tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) in the culture medium were determined by commercial assay kits after the cells treated with different concentrations of MPEE for 24 h. The mRNA expression levels of glucose transporter-4 (GLUT-4), hormone-sensitive triglyceride lipase(HSL) and inflammation related factors [including TNF-α, IL-6, monocyte chemotactic protein-1 (MCP-1) and C-reaction protein (CRP)] in cells were determined by Quantitative real-time PCR (qRT-PCR) assay. MPEE could enhance the glucose consumption, decrease the cellular TG levels and reduce the releasing of FFA and glycerol in insulin resistance cells. MPEE also reduced the secretion of TNF-α and IL-6, and reduced the mRNA expressions of inflammation mediators (including TNF-α, IL-6, MCP-1 and CRP) in model cells. In addition, MPEE was able to up-regulate the mRNA levels of GLUT-4, and reduce the mRNA levels of HSL in insulin resistance cells. These results suggested that the MPEE treatment was able to regulate the glucose and lipid metabolism to against the insulin resistance via increasing the glucose consumption, enhancing the TG lipolysis, and reducing the releasing of FFA and glycerol in 3T3-L1 adipocytes. MPEE treatment also down-regulated the inflammation levels by reducing the secretion of inflammatory mediators.

广西壮族自治区教育厅中青年教师基础能力提升项目（KY2016YB328、KY2016YB321）；广西壮族自治区"大学生创新创业训练计划"资助项目（201510601076）；桂林医学院引进人才科研启动基金项目（04010150001）