柳蘑多糖对肿瘤细胞增殖抑制作用的研究

Inhibitory Effect of Polysaccharides from Pholiota adiposa on Tumor Cell Proliferation

摘要:

为了研究提取溶剂酸碱性对柳蘑多糖的制备及抑制肿瘤细胞增殖的影响，本文利用稀酸、蒸馏水和稀碱溶剂分别制备三种柳蘑多糖（SPAP1、WPAP1和JPAP1），经过脱蛋白对应得到三种脱蛋白的柳蘑多糖（SPAP2、WPAP2和JPAP2），分别考察柳蘑多糖提取率和纯度的变化以及对肿瘤细胞增殖抑制作用的影响，并采用红外光谱仪初步分析柳蘑多糖的结构差异。研究表明，提取溶剂酸碱性对柳蘑多糖的提取率及纯度均有显著影响，其中JPAP1的提取率最高，为11.02%，SPAP2的纯度最高，为73.91%。SPAP2对肿瘤细胞增殖抑制效果显著高于其它柳蘑多糖（p<0.05），并与多糖浓度和给药时间成量效关系。其中SPAP2对Hela细胞的增值抑制效果最好，抑制率为90.88%；红外光谱分析SPAP2在1078±4 cm-1和759.83±5 cm-1处有较强的吸收峰，表明SPAP2中C-O-H或C-O-C和环氧基团含量高于其它柳蘑多糖。本论文结果为深入开发柳蘑多糖产品及进一步研究柳蘑多糖诱导肿瘤细胞凋亡机制提供理论依据。

Abstract:

To study the effects of extraction solvent acidity on the preparation of Pholiota adiposa polysaccharides (e.g., yield and purity) and their inhibitory effects on tumor cell proliferation, three types of P. adiposa polysaccharides (SPAP1, WPAP1, and JPAP1) were prepared using 0.1 mol/L HCl (aq), distilled water, and 0.1 mol/L sodium hydroxide (aq), respectively. The deproteinized P. adiposa polysaccharides (SPAP2, WPAP2, and JPAP2) were obtained from the deproteinization of SPAP1, WPAP1, and JPAP1, respectively. The effects of the extraction yield and purity of P. adiposa polysaccharides on the proliferation of tumor cells were investigated, and the differences in the structures of P. adiposa polysaccharides were preliminarily analyzed by infrared spectrometry. The result indicated that the acidity of the extraction solvent considerably impacted the extraction yield and purity of P. adiposa polysaccharides, and the highest extraction yield and the highest purity were found in JPAP1 (11.02%) and SPAP2 (73.91%), respectively. The inhibitory effect of SPAP2 on tumor cell proliferation was significantly higher than those of other P. adiposa polysaccharides (p<0.05) and dose- and time-dependent effects of polysaccharide treatment were observed. SPAP2 exhibited thestrongest inhibitory effect on the proliferation of HeLa cells, with an inhibition rate of 90.88%. Infrared spectroscopy results showed that SPAP2 had strong absorption peaks at 1078±4 cm-1 and 759.83 cm-1, which indicated that the content of C-O-H and C-O-C groups in SPAP2 were higher than those in other polysaccharides. These results may provide a theoretical basis for further development of P. adiposa polysaccharide products and an in-depth study on the mechanism of P. adiposa polysaccharide-induced tumor cell apoptosis.