绿蒜正己烷萃取物对H22荷瘤小鼠的影响

Effects of n-Hexane Extract of Green Garlic on H22 Tumor-bearing Mice

摘要:

为了探讨绿蒜75%乙醇提取物的正己烷萃取物（简称绿蒜正己烷萃取物：GGHE）对H22荷瘤小鼠的抑瘤作用及对其免疫功能的影响，建立H22荷瘤小鼠模型。将60只小鼠平均分为6组：空白对照组、模型组、阳性对照组（环磷酰胺CTX，20 mg/kg）、GGHE低中高剂量组（L-GGHE：75 mg/kg；M-GGHE：150 mg/kg；H-GGHE：300 mg/kg)，连续给药14 d。测定小鼠体重、水食量、肝脏中SOD、GSH-Px、CAT、ALT、AST的活性和MDA的含量；测定小鼠血清中细胞因子TNF-α、IL-2和VEGF的含量；计算水食效率、瘤体积、抑瘤率、脏器指数；分析肿瘤组织的病理特征。实验结果表明：M-GGHE的抑瘤率达71.48%。与模型组相比，GGHE能显著提高小鼠水食效率、胸腺及脾脏指数以及IL-2，TNF-α的水平，降低VEGF的表达。不同程度的提高肝脏组织中抗氧化酶SOD、GSH-Px和CAT的活力、降低MDA的含量以及转氨酶ALT和AST的活力。结论：GGHE对H22荷瘤小鼠的肿瘤生长有一定的抑制作用，改善了荷瘤小鼠的生存质量，提高了荷瘤小鼠的抗氧化水平和免疫功能。本实验提示可将绿蒜75%乙醇提取物的正己烷萃取物开发成治疗肝癌的候选药物。

Abstract:

To study the antitumor effects of the hexane extract derived from 75% ethanol green garlic extract (hereafter referred to as green garlic n-hexane extracts: GGHE) and its effect on immune function in H22 tumor-bearing mice, H22 transplanted mouse models were established. Sixty mice were divided into six groups: blank control group, model group, positive control (cyclophosphamide (CTX): 20 mg/kg), and low-, medium-, and high-dose GGHE (L-GGHE: 75 mg/kg, M-GGHE: 150 mg/kg, and H-GGHE: 300 mg/kg) groups. The drugs were continuously administered for 14 days. Mouse body weight; food/water intake; the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase (CAT), alanine transaminase (ALT), and aspartate aminotransferase (AST); and the content of malondialdehyde (MDA) in liver tissue were determined. Moreover, the levels of tumor necrosis factor (TNF)-α, interleukin (IL)-2, and vascular endothelial growth factor (VEGF) in serum were detected. The food and water efficiency, tumor volume, tumor inhibition rate, and spleen and thymus indices were calculated. The pathological characteristics of tumor tissues were also analyzed. The results showed that the tumor growth inhibition rate of M-GGHE was 71.48%. Compared with the model group, the treatment with GGHE significantly increased the food and water efficiency rate, spleen and thymus indices, increased the levels of IL-2 and TNF-α; reduced the level of VEGF; increased the activities of SOD, GSH-Px, and CAT, and decreased the MDA content as well as the activities of ALT and AST. In conclusion, GGHE exhibited antitumor effects on H22 tumor-bearing mice, improved their quality of life, and improved the antioxidant level and immune function. The results suggest that GGHE could be used as a candidate drug for treating hepatocellular carcinoma.