本文采用SD大鼠作为试验对象，以棉球植入SD大鼠建立炎症模型，研究植物组方提取物M对SD大鼠及棉球植入所致肉芽肿炎症模型SD大鼠的体重、摄食量和饮水量的影响，采用酶联免疫试剂盒（ELISA）研究其对棉球植入所致肉芽肿炎症模型SD大鼠的炎症介质分泌量以及前列腺素E2、白三烯的合成途径的影响。结果发现：植物组方提取物M对正常SD大鼠的体重、摄食量和饮水量无显著影响，对建立炎症模型后的大鼠摄食量有一定影响；植物组方提取物M能够显著缓解炎症反应中过量分泌C反应蛋白、一氧化氮、花生四烯酸5脂氧合酶和白三烯C4，对白介素-1β、肿瘤坏死因子-α、前列腺素E2和环加氧酶2的分泌无显著抑制作用，说明植物组方提取物M能够通过抑制花生四烯酸5脂氧合酶合成白三烯C4发挥抗炎作用，具有一定的抗炎应用价值。

Sprague–Dawley (SD) rats were used as the experimental model in this paper whereby an SD rat model of inflammation was established by implanting cotton ball. Further, the effects of extract M of plant combinations on the body weight, food intake, and water intake of rats with cotton-ball-induced inflammation were studied. Enzyme-linked immunosorbent assay (ELISA) was employed to study the effects of extract M of plant combinations on the secretion of inflammatory mediators and synthetic pathways of prostaglandin E2 and leukotriene in rats with cotton-ball-induced inflammation. The results showed that extract M had no significant effects on the body weight, food intake, and water intake of normal SD rats, however had a certain impact on the food intake of rats after the model of inflammation was established. Extract M could significantly alleviate the excessive secretions of C-reactive protein, nitric oxide, arachidonate 5-lipoxygenase, and leukotriene C4 in the inflammation reaction, and had no significant inhibitory effects on the secretions of interleukin-1β, tumor necrosis factor-α, prostaglandin E2, and cyclooxygenase 2. The above findings indicated that extract M of plant combinations exerted anti-inflammatory effects by inhibiting the synthesis of leukotriene C4 by arachidonate 5-lipoxygenase and therefore can contribute to anti-inflammatory applications.

广东省自然科学基金项目（2014A030313242）；中央高校科研业务费项目（2015ZZ110）