Abstract:In order to evaluate the effects of probiotics on the restoration of intestinal microflora in a mouse model of diarrhea, 22.4 g/(kg·d) ampicillin was administered by gavage to construct the mouse model of diarrhea, and the blank control group was given saline at the same dosage. After the model was successfully constructed, low-, medium-, and high-dose probiotics were used to treat the mice to restore their intestinal microflora. The effect of probiotics on the loose stool rate, degree of loose stool, and diarrhea index were observed after 14 days of treatment. The aseptically collected fecal samples of mice at days 0, 7, and 14 after treatment were analyzed, and changes in the target intestinal flora were determined using denaturing gradient gel electrophoresis (DGGE) and plate counting methods. After the completion of probiotic treatment, the fecal samples of mice were collected aseptically at days 0, 7, and 14, and real-time fluorescent quantitative PCR (FQ-PCR) was carried out for the quantitative determination of exogenous probiotics. The results showed that probiotics not only mitigated diarrhea in mice and promoted mouse growth, but also exhibited a significant effect on the restoration of intestinal flora in mice with diarrhea. The results from FQ-PCR analysis indicated that the exogenous probiotics Lactobacillus acidophilus NCFM, Bifidobacterium lactis Bi-07, and Lactobacillus rhamnosus HN001 were able to colonize and survive after the administration of probiotics was stopped, but showed a certain downward trend over time.
