活体运输过程中冰片麻醉剂与MS-222对草鱼麻醉效果的对比研究

Comparative Study of the Anesthetic Effects of Borneol Anesthetic and MS-222 in Simulated Transportation of Live Ctenopharyngodon idellus

摘要:

为提高水产品活体运输的存活率，文章探明了将传统中药冰片作为麻醉剂应用于水产品活体运输的可能性。对比了不同浓度的MS-222（间氨基苯甲酸乙酯甲磺酸盐，也称“鱼安定”）和冰片麻醉剂对草鱼(Ctenopharyngodon idellus)的麻醉效果，并在30 mg/L的冰片麻醉剂和20 mg/L的MS-222中研究了水温对麻醉效果的影响，最后在20 ℃水温条件下分别在30 mg/L的冰片麻醉剂和20 mg/L的MS-222中进行了麻醉模拟运输试验并对关键生化指标进行比较分析。麻醉试验结果表明：随麻醉剂浓度增加，草鱼进入相同麻醉期时间缩短，完全复苏时间延长，20~40 mg/L的冰片麻醉剂适于草鱼的麻醉运输。麻醉时间随水温降低而缩短，复苏时间随着水温降低而增加，20~25 ℃水温适于草鱼的冰片麻醉运输。麻醉模拟运输试验结果表明：冰片麻醉运输24 h存活率为100%，MS-222麻醉运输24 h存活率为70%。复苏时间随运输时间的增加而增加。冰片麻醉运输各项生化指标均优于MS-222麻醉运输。文章结果显示，在20~25 ℃水温条件下，冰片麻醉运输保活效果优于MS-222。

Abstract:

To improve the survival rate of aquatic animals during live transport, the feasibility of traditional Chinese medicine borneol as an anesthetic was explored in this study. The anesthetic effects of borneol and MS-222 (tricaine methanesulfonate or fish diazepam) at different concentrations on Ctenopharyngodon idellus and the impact of water temperature on its anesthetic effects were studied following administration of 30 mg/L borneol and 20 mg/L MS-222. Additionally, simulated post-anesthesia transport was conducted at 20 ℃ after administration of 30 mg/L borneol and 20 mg/L MS-222, followed by comparative analysis of key biochemical indicators. Anesthesia test results showed that rising anesthetic concentrations shortened the time necessary for C. idellus to reach the same anesthetic depth, prolonged full-recovery time, and A concentration range between ~20 mg/L and 40 mg/L borneol was suitable for C. idellus anesthesia transport. Additionally, in the presence of decreasing water temperature, anesthesia time was shortened, but recovery time was increased, and a water temperature between ~20 ℃ and 25 ℃ was suitable for C. idellus transport. Simulated anesthesia-transportation test results showed that C. idellus survival rates after 24 h of transport following borneol or MS-222 administration were 100% and 70%, respectively. Furthermore, recovery time increased as transport duration increased, and all biochemical indicators associated with transport following borneol administration were better as compared with those following MS-222 administration. Therefore, the results of this study suggested that the effect of anesthesia-related transport using borneol on preservation was superior to that using MS-222 at a water temperature between 20 ℃ and 25 ℃.