本文采用修正的Hurnmers方法制备了氧化石墨，通过超声波剥离得到氧化石墨烯溶液，用水热法进行还原，制得三维石墨烯。结果表明，制备的三维石墨烯材料具有多孔结构，且还原后某些含氧基团消失。所构建的酶生物燃料电池以三维石墨烯(GN)为载体，以麦尔多拉蓝(MB)为导电介体，用Nafion(NF)固定甘油激酶(GK)和甘油-3-磷酸氧化酶(GPO)制备阳极催化电极，并以Pt/C作为阴极催化剂。结果显示，基于NF/GN/MB酶修饰碳纸电极的甘油酶生物燃料电池具有良好的放电性能，开路电位达到0.77 V，并在0.42 V取得最大功率密度42.05 μW/cm2。论文中对该酶生物燃料电池的组装、工作条件等进行了优化，用极化曲线法和交流阻抗法对其性能进行了评价。该酶生物燃料电池将为生物柴油副产物甘油的处理，提供一种清洁可靠的能源再利用方式。

Graphene oxide (GO) was prepared according to a modified Hummer’s method, and graphene oxide aqueous solution, obtained by ultrasonic exfoliation, was then reduced by hydrothermal method to produce three-dimensional graphene (GN). The results showed that the as-prepared three-dimensional graphene material possessed a porous structure and some oxygen-containing groups disappeared after reduction. A glycerol enzymatic biofuel cell (EBFC) was prepared using three-dimensional GN as the carrier, and Meldola’s blue as the mediator; Nafion (NF) was used to immobilize glycerol kinase (GK) and glycerol-3-phosphate oxidase (GPO) to prepare the anode catalyst, and platinum on carbon was used as the cathode catalyst. The results showed that the glycerol EBFC based on the NF/GN/MB enzyme-modified carbon paper electrode had good discharge performance. The open circuit potential was 0.77 V and the maximum power density was 42.05 μW/cm2 at 0.42 V. The assembly of the glycerol EBFC and the working conditions were optimized. The performance was also evaluated using polarization curve and alternating current (AC) impedance methods. This EBFC will provide a clean and reliable energy reuse approach for the treatment of glycerol, a by-product of biodiesel in the future.

国家自然科学基金项目（21576101、21476087、21676106）；十三五国家重点研发计划子课题（2016YFB0101200、2016YFB0101204）；中央高校基本科研业务费