白簕茎多糖对链脲佐菌素致糖尿病小鼠的降血糖作用研究

Hypoglycemic Effect of Polysaccharide from Acanthopanax Trifoliatus (Linn.) Merr. Stem in Streptozotocin-induced Diabetic Mice

摘要:

本实验旨在研究白簕茎粗多糖（ATMP）对链脲佐菌素（STZ）致糖尿病小鼠的降血糖效果及其机制。采用STZ造模实验性糖尿病小鼠，分为正常组、高血糖模型组、二甲双胍组、ATMP低、中、高剂量组，灌胃相应的药物4周后，分别测定体重、脏器重量、血糖等指标，并进行肾脏、脾脏等的HE染色观察。结果显示连续给药4周后，100 mg/(kg?d)及200 mg/(kg?d)的ATMP可使STZ诱导糖尿病小鼠的血糖分别下降47.73%和53.52%；有效改善糖尿病小鼠糖耐量，与高糖模型组相比，ATMP中高剂量组小鼠的AUC明显降低，同时该两组小鼠的胸腺指数也显著增加，比高糖模型组高48.9%和51.1%。HE染色观察后发现4周给药ATMP可对胰腺有不同程度的治疗效果，可增强小鼠脾脏免疫功能。研究结果表明，ATMP在STZ造模糖尿病小鼠体内具有降血糖的作用，且该作用可能与调节免疫有关。

Abstract:

The anti-hyperglycemic effect of polysaccharides from Acanthopananx trifoliatus (L.) Merr. stem (ATMP) was investigated in streptozotocin (STZ)-induced diabetic mice. These mice were divided into six groups, a normal group, a high blood glucose group, a metformin group, an ATMP low-dose [50 mg/(kg?d)] group, an ATMP middle-dose [100 mg/(kg?d)] group, and an ATMP high-dose [200 mg/(kg?d)] group. Four weeks after dosing by intra-peritoneal injection, body weight, organ index, blood glucose, and oral glucose tolerance were examined for all groups, and morphological changes were determined by HE staining. The polysaccharides at doses of 100 mg/(kg?d) and 200 mg/(kg?d) decreased blood glucose significantly by 47.73% and 53.52%, respectively, and increased oral glucose tolerance. The AUC of mice in the 100 mg/(kg?d) and 200 mg/(kg?d) ATMP group were significantly lower than those in the high blood glucose group. ATMP at these doses also increased the thymus index by 48.9% and 51.1%, respectively. HE staining showed that ATMP had a therapeutic effect on the pancreas and spleen. The results showed that ATMP has an anti-hyperglycemic effect, and this may occur through some form of immune regulation.