自制研发奶粉对ox-LDL诱导平滑肌细胞增殖的影响

Effect of Self-developed Milk Powder on Oxidized-low Density Lipoprotein (ox-LDL)-Induced Proliferation of Vascular Smooth Muscle Cells

摘要:

研究自制研发的中老年心血管奶粉对氧化型低密度脂蛋白（ox-LDL）诱导的平滑肌细胞增殖与NO释放量的影响。用ox-LDL诱导血管平滑肌细胞（vascular smooth muscle cell，VSMC）增殖，建立VSMC增殖模型，通过比较自制奶粉与3种常见市售奶粉对模型细胞的增殖抑制作用和NO释放量的数据结果，评价实验室自制研发奶粉的功效。结果表明，30 μg/mL的ox-LDL作用VSMC 24 h可极显著的促进细胞增殖并抑制NO的释放(p<0.01)，说明模型构建成功；在此VSMC增殖模型中分别添加奶粉样品，得出自制奶粉在200、400、800 μg/mL均能抑制ox-LDL诱导的VSMC的增殖，且在400 μg/mL的抑制作用极显著（p<0.01），并均能极显著的促进NO的释放（p<0.01）。说明自制研发的中老年心血管奶粉能够抑制ox-LDL诱导的VSMC增殖模型，具有较好的预防动脉粥样硬化的功效。

Abstract:

The effect of self-developed milk powder on the proliferation and nitric oxide (NO) production of vascular smooth muscle cells (VSMC) induced by oxidized-low density lipoprotein (ox-LDL) was studied. A VSMC proliferation model was established by inducing the proliferation of VSMC with ox-LDL. The effects of the milk powder developed in our laboratory was evaluated by comparing its inhibitory effect on the proliferation and NO production of the model cells with three kinds of common commercial milk powders. The results indicated that when VSMCs were treated with 30 μg/mL ox-LDL for 24 h, cell proliferation was significantly promoted and NO release was inhibited (p<0.01), indicating that the model was successfully established. In this VSMC model, different amounts of self-developed milk powder were added, and the results showed that solutions of 200, 400, and 800 μg/mL milk powder inhibited ox-LDL-induced VSMC proliferation. In addition, 400 μg/mL milk powder highly inhibited cell proliferation and promoted NO production (p<0.01). The above findings demonstrate that self-developed milk powder inhibited the ox-LDL-induced proliferation in VSMCs; therefore these milk powders may prevent atherosclerosis and be beneficial for middle-aged and older populations.