杏鲍菇菌丝体多肽的免疫活性及抗肿瘤作用

Immunological and Antitumor Activities of Polypeptides from Pleurotus eryngii Mycelium

摘要:

为研究杏鲍菇菌丝体多肽的免疫活性及抗肿瘤作用，以杏鲍菇菌丝体多肽为原料，通过对小鼠巨噬细胞Ana-1细胞存活率、细胞因子TNF-α和IL-6分泌量、膜表面蛋白TLR2和TLR4表达量、吞噬中性红活性以及刺激巨噬细胞产生NO和H2O2的能力测定来研究杏鲍菇菌丝体多肽的免疫活性；通过对人体乳腺癌细胞BT-549细胞、宫颈癌细胞Hela-229和胃癌细胞HGC-27细胞增殖的抑制来研究杏鲍菇菌丝体多肽的抗肿瘤作用。实验显示，多肽浓度在0.05~2 mg/mL范围时，在一定程度上能够促进巨噬细胞的增殖，提高膜表面蛋白TLR2、TLR4表达量，提高细胞因子TNF-α和IL-6分泌量，增强巨噬细胞吞噬中性红活性能力，并且促进巨噬细胞分泌NO和H2O2；对人体乳腺癌细胞、宫颈癌细胞和胃癌细胞这三种癌细胞具有较强的抑制作用，表明杏鲍菇菌丝体多肽具有较好的免疫活性和抗肿瘤活性，且活性与浓度呈现量效关系。

Abstract:

The aim of the present work was to study the immunological and antitumor activities of polypeptides from Pleurotus eryngii mycelium, which was used as the raw material. The immunological activities of the polypeptides were investigated by measuring neutral red uptake capacity, the ability to stimulate macrophages to secret nitric oxide and hydrogen peroxide, the levels of the secreted cytokines tumor necrosis factor-α and interleukin-6, the expression levels of the membrane surface proteins toll-like receptor (TLR)2 and TLR4, and Ana-1 cell survival rate. The antitumor activities of the polypeptides were studied through the inhibition of the proliferation of human breast cancer cells (BT-549), cervical cancer cells (Hela-229), and human gastric cancer cells (HGC-27). The results showed that within a concentration range of 0.05~2 mg/mL, the Pleurotus eryngii mycelium polypeptide could, to some extent, promote the proliferation of macrophages, enhance TLR2 and TLR4 expression levels and macrophage neutral red uptake, and increase the secretion of nitric oxide and hydrogen peroxide by macrophages. The polypeptide had strong inhibitory effects on the proliferation of the breast cancer, cervical cancer, and human gastric cancer cells. These results indicate that the polypeptides from Pleurotus eryngii mycelium had good immunological and antitumor activities with a concentration-response relationship.