沙虫蛋白酶解产物抗氧化与免疫调节活性的研究
CSTR:
作者:
作者单位:

作者简介:

孙雪萍(1982-),女,博士,助理研究员,研究方向:天然产物化学 通讯作者:孙振亮(1984-),男,博士,助理研究员,研究方向:天然产物化学

通讯作者:

中图分类号:

基金项目:

广西壮族自治区海洋生物技术重点实验室开放课题(GLMBT-201407);广西中药质量标准研究重点实验室自主研究课题(桂中重系201501);国家自然科学基金资助项目(41306133);上海市奉贤区科委重点项目(20141001)


Antioxidant and Immunomodulatory Activities of the Protein Hydrolysate from Sipunculus nudus
Author:
Affiliation:

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    采用复合蛋白酶对沙虫(Sipunculus nudu)进行酶解,得到沙虫蛋白酶解产物(SNPH),并对其氨基酸组成进行了分析。采用高效液相凝胶色谱法对SNPH的相对分子质量分布进行了测定。采用紫外分光光度法测定SNPH对DPPH自由基,羟基自由基、超氧阴离子的清除能力及其还原能力。采用四甲基偶氮唑盐(MTT)法检测SNPH在对刀豆蛋白(Con A)和脂多糖(LPS)诱导的小鼠脾淋巴细胞增殖作用的影响。结果显示SNPH中必需氨基酸和疏水氨基酸含量较高,小分子肽含量较高,相对分子质量在120~1800之间的物质占85.11%。抗氧化活性实验显示SNPH对DPPH自由基,羟基自由基和超氧阴离子具有较强的清除效果,且清除率与浓度存在一定的量效关系。免疫调节活性试验表明SNPH对Con A和LPS诱导的小鼠脾淋巴细胞的增殖具有协同刺激作用。SNPH具有较好的抗氧化和免疫调节活性。

    Abstract:

    Enzymatic hydrolysis of Sipunculus nudus was performed using Protamex to obtain SNPH. Subsequently, the amino acid composition of SNPH was analyzed, and the distribution of relative molecular mass was determined using a high performance gel chromatography method. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity, hydroxyl radical scavenging activity, superoxide anion scavenging activity, and reducing power of SNPH were evaluated using ultraviolet (UV) spectrophotometry. The effects of SNPG treatment on the splenic lymphocyte proliferation induced by concanavalin (Con) A and lipopolysaccharide (LPS) in mice were determined using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The results indicated that SNPH had high contents of essential amino acids and hydrophobic amino acids. The molecular weight distribution showed that SNPH had a high content of oligopeptides, and the substances with a relative molecular mass of 120~1800 accounted for 85.11%. The antioxidant activity experiment revealed that SNPH possessed potent scavenging activities on DPPH free radical, hydroxyl free radical, and superoxide anion in a good dose-effect relationship. The experiment on immunomodulatory activity indicated that SNPH could have a synergistic effect on the splenic lymphocyte proliferation induced by Con A and LPS. SNPH had good antioxidant and immunomodulatory activities.

    参考文献
    相似文献
    引证文献
引用本文

孙雪萍,徐艳,刘布鸣,孙振亮,刘新.沙虫蛋白酶解产物抗氧化与免疫调节活性的研究[J].现代食品科技,2017,33(4):67-72.

复制
相关视频

分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:2015-11-19
  • 最后修改日期:
  • 录用日期:
  • 在线发布日期: 2017-04-28
  • 出版日期:
文章二维码
×
因办公室装修,期间暂时无法接听电话,如有事请QQ或邮件联系。信息咨询:QQ: 2553003667稿件处理1:QQ: 1542354573稿件处理2:QQ: 2195608851 财务咨询:QQ: 1347040116 Email:mfood@scut.edu.cn、mfood@foxmail.com